A new method to process testicular sperm: combining enzymatic digestion, accumulation of spermatozoa, and stimulation of motility

Purpose In azoospermia processing of the TESE material often results in a sample of reduced purity. This prospective study was set up to clarify whether a combination of enzymatic digestion, density gradient centrifugation and stimulation of motility (where indicated) is a feasible option in TESE pa...

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Bibliographic Details
Published in:Archives of gynecology and obstetrics 2015-03, Vol.291 (3), p.689-694
Main Authors: Wöber, Martina, Ebner, Thomas, Steiner, Sarah L., Strohmer, Heinz, Oppelt, Peter, Plas, Eugen, Obruca, Andreas
Format: Article
Language:English
Subjects:
Adult
Azoospermia
Birth Rate
Cell Movement
Cryopreservation
Endocrinology
Female
Gynecologic Endocrinology and Reproductive Medicine
Gynecology
Human Genetics
Humans
Infertility, Male - therapy
Male
Medicine
Medicine & Public Health
Middle Aged
Motility
Obstetrics/Perinatology/Midwifery
Pregnancy
Pregnancy Outcome
Pregnancy Rate
Prospective Studies
Sperm
Sperm Injections, Intracytoplasmic - methods
Sperm Motility
Sperm Retrieval
Spermatozoa - physiology
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Summary:Purpose In azoospermia processing of the TESE material often results in a sample of reduced purity. This prospective study was set up to clarify whether a combination of enzymatic digestion, density gradient centrifugation and stimulation of motility (where indicated) is a feasible option in TESE patients. Methods A total of 63 samples (showing spermatozoa) were processed by the present tripartite processing method. The resulting sperm sample of high purity was directly used for ICSI and subsequent cryopreservation when quality of the accumulated sperm sample allowed for it ( n  = 39 cycles). Results Compared to the control group blastocyst formation rate in the present tripartite processing technique was significantly ( P  
ISSN:0932-0067
1432-0711
DOI:10.1007/s00404-014-3458-3