Gingival tissue healing following Er:YAG laser ablation compared to electrosurgery in rats

• Published in: Lasers in medical science 2015-02, Vol.30 (2), p.875-883
• Main Authors: Sawabe, Masanori, Aoki, Akira, Komaki, Motohiro, Iwasaki, Kengo, Ogita, Mayumi, Izumi, Yuichi
• Format: Article
• Language: English
• Subjects: Aluminum; Animals; Comparative analysis; Dental Cements - radiation effects; Dental Enamel - pathology; Dental Enamel - radiation effects; Dentistry; Electrosurgery; Gingiva - pathology; Gingiva - radiation effects; Gingiva - surgery; Immunohistochemistry; Laser Therapy; Lasers; Lasers, Solid-State; Male; Medicine; Medicine & Public Health; Optical Devices; Optics; Original Article; Photonics; Quantum Optics; Rats, Sprague-Dawley; Re-Epithelialization - radiation effects; Rodents; Surgery; Tissues; Wound healing; Wound Healing - radiation effects; Yttrium
• ISSN: 0268-8921; 1435-604X
• DOI: 10.1007/s10103-013-1478-z

The erbium-doped yttrium aluminum garnet (Er:YAG) laser is currently used for periodontal soft tissue management with favorable outcomes. However, the process of wound healing after Er:YAG laser (ErL) treatment has not been fully elucidated yet. The aim of this study was to investigate the gingival tissue healing after ErL ablation in comparison with that after electrosurgery (ElS). Gingival defects were created in 28 rats by ablation with ErL irradiation or ElS. The chronological changes in wound healing were evaluated using histological, histometrical, and immunohistochemical analyses. The ErL-ablated gingival tissue revealed much less thermal damage, compared to the ElS. In the ElS sites, the postoperative tissue destruction continued due to thermal damage, while in the ErL sites, tissue degradation was limited and the defects were re-epithelialized early. Heat shock protein (Hsp) 72/73 expression was detected abundantly remote from the wound in the ElS, whereas it was slightly observed in close proximity to the wound in the ErL sites. Hsp47 expression was observed in the entire connective tissue early in the wound healing and was found limited in the wound area later. This phenomenon proceeded faster in the ErL sites than in the ElS sites. Expression of proliferating cell nuclear antigen (PCNA) persisted in the epithelial tissue for a longer period in the ElS than that in the ErL. The ErL results in faster and more favorable gingival wound healing compared to the ElS, suggesting that the ErL is a safe and suitable tool for periodontal soft tissue management.