Establishment of the cells useful for murine interleukin-18 bioassay by introducing murine interleukin-18 receptor cDNA into human myelomonocytic KG-1 cells

We genetically engineered human myelomonocytic KG-1 cells by introducing cDNA of murine interleukin-18 receptor (MuIL-18R) and established human cells which were capable of responding to MuIL-18. These cells expressed larger number of MuIL-18R (>13,000 sites/cell) than intrinsic human IL-18 recep...

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Bibliographic Details
Published in:Journal of immunological methods 1998-08, Vol.217 (1), p.97-102
Main Authors: Taniguchi, Mutsuko, Nagaoka, Katsue, Ushio, Shimpei, Nukada, Yoshiyuki, Okura, Takanori, Mori, Tsutomu, Yamauchi, Hiroshi, Ohta, Tsunetaka, Ikegami, Hakuo, Kurimoto, Masashi
Format: Article
Language:English
Subjects:
Animal cells
Animals
Bioassay
Biological and medical sciences
Biological Assay
Cell cultures. Hybridization. Fusion
Cytokine
DNA, Complementary - genetics
Endotoxemia - blood
Endotoxemia - chemically induced
Endotoxemia - immunology
Fundamental and applied biological sciences. Psychology
Genetic Vectors
Human cells
Humans
Interferon-gamma - biosynthesis
Interleukin-18 - analysis
Interleukin-18 - metabolism
Interleukin-18 Receptor alpha Subunit
Lipopolysaccharides - toxicity
Mice
Mice, Inbred C57BL
Molecular and cellular biology
Monocytes - metabolism
Murine IL-18
Propionibacterium acnes
Protein Binding
Receptors, Interleukin - genetics
Receptors, Interleukin - metabolism
Receptors, Interleukin-18
Recombinant DNA
Recombinant Fusion Proteins - metabolism
Signal Transduction
Tumor Cells, Cultured
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Summary:We genetically engineered human myelomonocytic KG-1 cells by introducing cDNA of murine interleukin-18 receptor (MuIL-18R) and established human cells which were capable of responding to MuIL-18. These cells expressed larger number of MuIL-18R (>13,000 sites/cell) than intrinsic human IL-18 receptor (HuIL-18R) (
ISSN:0022-1759
1872-7905
DOI:10.1016/S0022-1759(98)00098-2