Octopine and nopaline strains of Agrobacterium tumefaciens differ in virulence; molecular characterization of the virF locus

Octopine and nopaline strains of Agrobacterium tumefaciens were found to differ in virulence on Nicotiana glauca . This difference is due to the absence of a functional virF locus, which is necessary for efficient tumorigenesis on N. glauca , from the nopaline Ti plasmids. Genetic studies and DNA se...

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Bibliographic Details
Published in:Plant molecular biology reporter 1990-01, Vol.14 (2), p.249-259
Main Authors: Melchers, L S, Maroney, MJ, den Dulk-Ras, A, Thompson, D V, van Vuuren, AJ, Schilperoort, R A, Hooykaas, PJJ
Format: Article
Language:English
Subjects:
Agrobacterium tumefaciens
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Summary:Octopine and nopaline strains of Agrobacterium tumefaciens were found to differ in virulence on Nicotiana glauca . This difference is due to the absence of a functional virF locus, which is necessary for efficient tumorigenesis on N. glauca , from the nopaline Ti plasmids. Genetic studies and DNA sequence analysis of the virF locus revealed that virF embraces one open reading frame coding for a hydrophilic protein with a molecular mass of 22,437 Da. Transcription of virF is directed from left to right, towards the T region, and is strongly induced by the phenolic compound acetosyringone.
ISSN:0735-9640