Octopine and nopaline strains of Agrobacterium tumefaciens differ in virulence; molecular characterization of the virF locus

Octopine and nopaline strains of Agrobacterium tumefaciens were found to differ in virulence on Nicotiana glauca . This difference is due to the absence of a functional virF locus, which is necessary for efficient tumorigenesis on N. glauca , from the nopaline Ti plasmids. Genetic studies and DNA se...

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Published in:Plant molecular biology reporter 1990-01, Vol.14 (2), p.249-259
Main Authors: Melchers, L S, Maroney, MJ, den Dulk-Ras, A, Thompson, D V, van Vuuren, AJ, Schilperoort, R A, Hooykaas, PJJ
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Agrobacterium tumefaciens
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container_title Plant molecular biology reporter
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creator Melchers, L S
Maroney, MJ
den Dulk-Ras, A
Thompson, D V
van Vuuren, AJ
Schilperoort, R A
Hooykaas, PJJ
description Octopine and nopaline strains of Agrobacterium tumefaciens were found to differ in virulence on Nicotiana glauca . This difference is due to the absence of a functional virF locus, which is necessary for efficient tumorigenesis on N. glauca , from the nopaline Ti plasmids. Genetic studies and DNA sequence analysis of the virF locus revealed that virF embraces one open reading frame coding for a hydrophilic protein with a molecular mass of 22,437 Da. Transcription of virF is directed from left to right, towards the T region, and is strongly induced by the phenolic compound acetosyringone.
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subjects Agrobacterium tumefaciens
title Octopine and nopaline strains of Agrobacterium tumefaciens differ in virulence; molecular characterization of the virF locus
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