Transforming growth factor beta sub(1) selectivity stimulates immunoglobulin G2b secretion by lipopolysaccharide-activated murine B cells

Bacterial lipopolysaccharide (LPS) has been reported to induce immunoglobulin (Ig)G2b class switching, yet we observed strain differences in IgG2b secretion in response to this mitogen. Specifically, BALB/c B cells, unlike those from DBA/2, synthesized relatively low amounts of IgG2b relative to IgG...

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Bibliographic Details
Published in:The Journal of experimental medicine 1993-01, Vol.177 (4), p.1031-1037
Main Authors: McIntyre, T M, Klinman, DR, Rothman, P, Lugo, M, Dasch, J R, Mond, J J, Snapper, C M
Format: Article
Language:English
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Summary:Bacterial lipopolysaccharide (LPS) has been reported to induce immunoglobulin (Ig)G2b class switching, yet we observed strain differences in IgG2b secretion in response to this mitogen. Specifically, BALB/c B cells, unlike those from DBA/2, synthesized relatively low amounts of IgG2b relative to IgG3, IgG1, or IgM. This report demonstrates that transforming growth factor (TGF) beta sub(1), previously shown to induce IgA class switching, selectively stimulates IgG2b secretion by BALB/c resting B cells activated with LPS. This activity was specifically reversed with a neutralizing anti-TGF- beta sub(1) antibody. We propose that TGF- beta sub(1) is a switch factor for the murine IgG2b subclass for appropriately activated B cells. In combination with other data, this would show that all six non-IgM, non-IgD isotypes in the mouse can be selectively induced by specific cytokines.
ISSN:0022-1007