Calmodulin-dependent protein phosphorylation during conidial germination and growth of Neurospora crassa

Conidial germination in Neurospora crassa was inhibited by the anticalmodulin (CaM) agents calmidazolium (CaMZ) and trifluoperazine (TFP), indicating the importance of CaM-dependent events during vegetative growth. An increase in intracellular CaM concentration, observed during conidial germination,...

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Bibliographic Details
Published in:Mycological research 1997-12, Vol.101 (12), p.1484-1488
Main Authors: RAO, J. PRAVEEN, REENA, G., SUBRAMANYAM, C.
Format: Article
Language:English
Subjects:
Biological and medical sciences
calmidazolium
calmodulin
chemical composition
conidia
Fundamental and applied biological sciences. Psychology
growth
Growth, nutrition, metabolism, transports, enzymes. Molecular biology
hyphae
inhibitors
Microbiology
molecular weight
Mycology
Neurospora crassa
phenylalanine
phosphorylation
spore germination
trifluoperazine
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Summary:Conidial germination in Neurospora crassa was inhibited by the anticalmodulin (CaM) agents calmidazolium (CaMZ) and trifluoperazine (TFP), indicating the importance of CaM-dependent events during vegetative growth. An increase in intracellular CaM concentration, observed during conidial germination, was associated with enhanced incorporation of [3H]phenylalanine into CaM and increased CaM-dependent protein kinase activity. Phosphorylation of two proteins (71 and 25 kDa) during conidial germination, and of four proteins (71, 47, 38 and 32 kDa) during hyphal elongation was noted to be CaM-dependent since these phosphorylations were inhibited in vitro by CaMZ. The possible regulatory functions of CaM during conidial germination are discussed in relation to phosphorylation of specific proteins during vegetative growth in N. crassa.
ISSN:0953-7562
1469-8102
DOI:10.1017/S0953756297004255