Development of a monoclonal antibody specific to cooked mammalian meats

Detection of species adulteration in ground meat products is important for consumer protection and food-labeling law enforcement. This study was conducted to develop monoclonal antibodies (MAbs) that can be used in an enzyme-linked immunosorbent assay (ELISA) for rapid detection of any cooked mammal...

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Bibliographic Details
Published in:Journal of food protection 1998-04, Vol.61 (4), p.476-481
Main Authors: Hsieh, Y.H.P. (Auburn University, Auburn, AL.), Sheu, S.C, Bridgman, R.C
Format: Article
Language:English
Subjects:
ADULTERACION
ADULTERATION
ANALYTICAL METHODS
Animals
Antibodies, Monoclonal - immunology
Antibody Specificity
ANTICORPS MONOCLONAL
ANTICUERPOS MONOCLONALES
Biological and medical sciences
Blotting, Western
Cattle
Cooking
DETECTION
Electrophoresis, Polyacrylamide Gel
Enzyme-Linked Immunosorbent Assay
Food Contamination
Food industries
Fundamental and applied biological sciences. Psychology
Mammals
Meat - analysis
Meat and meat product industries
MEAT PRODUCTS
Mice
MONOCLONAL ANTIBODIES
Poultry Products - analysis
PRODUCTOS DE LA CARNE
PRODUIT CARNE
RAPID METHODS
TECHNIQUE ANALYTIQUE
TECNICAS ANALITICAS
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Summary:Detection of species adulteration in ground meat products is important for consumer protection and food-labeling law enforcement. This study was conducted to develop monoclonal antibodies (MAbs) that can be used in an enzyme-linked immunosorbent assay (ELISA) for rapid detection of any cooked mammalian meats in cooked poultry products. Soluble muscle proteins extracted from cooked pork (heated at 100 degrees C for 15 min) were used as the antigen to immunize mice for developing the MAb. One that was developed, MAb 2F8 (IgG2b class), strongly reacted with cooked meat of five mammalian species (beef cattle, hogs, sheep, horse, and deer) but did not react with any cooked poultry (chicken, turkey, and duck) or raw meats. At least 0.5% by weight of pork, beef, lamb, and horse meats in a chicken-based mixture could be detected using an indirect ELISA with MAb 2F8. The MAb 2F8 is useful in a single initial screening test to detect the presence of five nonpoultry meat adulterants in cooked poultry products
ISSN:0362-028X
1944-9097
DOI:10.4315/0362-028X-61.4.476