A number of subgenomic DNAs are produced following agroinoculation of plants with beet curly top virus

Biotechnology Center and Department of Molecular Genetics, The Ohio State University, Columbus, Ohio 43210, U.S.A. In addition to ss and ds genomic DNA, agroinoculation of Nicotiana benthamiana plants with the Logan strain of the geminivirus beet curly top virus (BCTV) consistently resulted in de no...

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Bibliographic Details
Published in:Journal of general virology 1992-02, Vol.73 (2), p.237-242
Main Authors: Stenger, Drake C, Stevenson, Matthew C, Hormuzdi, Sheriar G, Bisaro, David M
Format: Article
Language:English
Subjects:
Base Sequence
beet curly top virus
Biological and medical sciences
Blotting, Southern
DNA Replication
DNA, Viral - analysis
DNA, Viral - biosynthesis
DNA, Viral - chemistry
Fundamental and applied biological sciences. Psychology
Genetics
Microbiology
Molecular Sequence Data
Nicotiana - microbiology
Nicotiana benthamiana
Plant Viruses - genetics
Plant Viruses - physiology
Plants, Toxic
Restriction Mapping
Virology
Virus Replication
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Summary:Biotechnology Center and Department of Molecular Genetics, The Ohio State University, Columbus, Ohio 43210, U.S.A. In addition to ss and ds genomic DNA, agroinoculation of Nicotiana benthamiana plants with the Logan strain of the geminivirus beet curly top virus (BCTV) consistently resulted in de novo production of subgenomic DNAs on initial passage. Single-stranded and dsDNA forms representing at least seven size classes (0.8 to 1.8 kb) of subgenomic DNA were observed in total DNA extracts from inoculated plants. Extracts from infected sugar beet and tomato contained variable but usually smaller amounts of subgenomic DNAs, suggesting that their production may be influenced by the host species. Restriction endonuclease mapping and partial nucleotide sequencing of three independent clones of a 1.5 kb size class indicated that this subgenomic DNA is produced from the standard viral genome by two separate deletion events. One deletion of 941 bp includes portions of the leftward open reading frames (ORFs) L1, L2 and L3, while the other deletion of 579 bp encompasses portions of the intergenic region and the rightward ORFs R1, R2 and R3. The data indicate that the 1.5 kb BCTV subgenomic DNA is a defective DNA that has retained ciselements essential for replication. Received 20 August 1991; accepted 16 October 1991.
ISSN:0022-1317
1465-2099
DOI:10.1099/0022-1317-73-2-237