LAP1 is a crucial protein for the maintenance of the nuclear envelope structure and cell cycle progression

Cell division in eukaryotes requires the disassembly of the nuclear envelope (NE) at the beginning of mitosis and its reassembly at the end of mitosis. These processes are complex and involve coordinated steps where NE proteins have a crucial role. Lamina-associated polypeptide 1 (LAP1) is an inner...

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Bibliographic Details
Published in:Molecular and cellular biochemistry 2015-01, Vol.399 (1-2), p.143-153
Main Authors: Santos, Mariana, Costa, Patrícia, Martins, Filipa, da Cruz e Silva, Edgar F., da Cruz e Silva, Odete A. B., Rebelo, Sandra
Format: Article
Language:English
Subjects:
Biochemistry
Biomedical and Life Sciences
Cardiology
Cell Cycle
Cell Line
Centrosome - metabolism
HSC70 Heat-Shock Proteins - physiology
Humans
Life Sciences
Medical Biochemistry
Microtubule-Organizing Center - metabolism
Nuclear Envelope - metabolism
Nuclear Envelope - ultrastructure
Oncology
Protein Transport
Tubulin - metabolism
Tubulins
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Summary:Cell division in eukaryotes requires the disassembly of the nuclear envelope (NE) at the beginning of mitosis and its reassembly at the end of mitosis. These processes are complex and involve coordinated steps where NE proteins have a crucial role. Lamina-associated polypeptide 1 (LAP1) is an inner nuclear membrane protein that has been associated with cell cycle events. In support of this role, LAP1 has been implicated in the regulation of the NE reassembly and assembly of the mitotic spindle during mitosis. In this study, we demonstrated that LAP1 intracellular levels vary during the cell cycle in SH-SY5Y cells, and that LAP1 is highly phosphorylated during mitosis. It is also clear that LAP1 co-localized with acetylated α-tubulin in the mitotic spindle and with γ-tubulin in centrosomes (main microtubule organizing center) in mitotic cells. Moreover, LAP1 knockdown resulted in decreased number of mitotic cells and decreased levels of acetylated α-tubulin (marker of microtubules stability) and lamin B1. Additionally, it was possible to determine that LAP1 is important for centrosome positioning near the NE. These findings place LAP1 at a key position to participate in the maintenance of the NE structure and progression of the cell cycle.
ISSN:0300-8177
1573-4919
DOI:10.1007/s11010-014-2241-x