Actinokineospora mzabensis sp. nov., a novel actinomycete isolated from Saharan soil

• Published in: Antonie van Leeuwenhoek 2015, Vol.107 (1), p.291-296
• Main Authors: Aouiche, Adel, Bouras, Noureddine, Mokrane, Salim, Zitouni, Abdelghani, Schumann, Peter, Spröer, Cathrin, Sabaou, Nasserdine, Klenk, Hans-Peter
• Format: Article
• Language: English
• Subjects: Actinobacteria - classification; Actinobacteria - genetics; Actinobacteria - isolation & purification; Actinobacteria - physiology; Actinokineospora inagensis; Africa, Northern; Algeria; arabinose; Bacterial Typing Techniques; Carbohydrates - analysis; Cell Wall - chemistry; Cluster Analysis; Cytosol - chemistry; Diaminopimelic Acid - analysis; DNA, Bacterial - chemistry; DNA, Bacterial - genetics; DNA, Ribosomal - chemistry; DNA, Ribosomal - genetics; fatty acids; Fatty Acids - analysis; galactose; Hydrogen-Ion Concentration; hydrolysates; menaquinones; Molecular Sequence Data; mycelium; new species; nucleotide sequences; phosphatidylethanolamines; Phospholipids - analysis; Phylogeny; ribosomal RNA; RNA, Ribosomal, 16S - genetics; Sequence Analysis, DNA; soil; Soil Microbiology; Temperature; Vitamin K 2 - analysis
• ISSN: 0003-6072; 1572-9699
• DOI: 10.1007/s10482-014-0328-8

A novel actinomycete strain, designated PAL84, was isolated from a Saharan soil sample collected from Béni-Isguen, Ghardaïa (South of Algeria). This strain was studied for its taxonomic position using a polyphasic approach and was identified as a member of the genus Actinokineospora. Phylogenetic analysis showed that strain PAL84 had 16S rRNA gene sequence similarities with members of the genus Actinokineospora ranging from 96.2 % (Actinokineospora inagensis DSM 44258ᵀ) to 97.8 % (Actinokineospora baliensis NBRC 104211ᵀ). The strain was observed to produce pinkish-purple aerial mycelium and purplish red substrate mycelium, which fragmented readily into chains of non-motile elements. The optimum growth temperature and pH were found to be 25–30 °C and 5.0–7.0, respectively. The cell-wall hydrolysate of strain PAL84 was found to contain meso-diaminopimelic acid and the diagnostic whole-cell sugars were identified as arabinose and galactose. The predominant menaquinone was identified as MK-9 (H₄). The major fatty acids were found to be iso-C₁₆:₀, iso-C₁₅:₀, iso-C₁₆:₁H and iso-C₁₆:₀2OH. The diagnostic phospholipid detected was phosphatidylethanolamine. The genotypic and phenotypic data show that the strain represents a novel species of the genus Actinokineospora, for which the name Actinokineospora mzabensis sp. nov. is proposed, with the type strain PAL84ᵀ(=DSM 45961ᵀ = CECT 8578ᵀ).