Sensitive and rapid detection of Mycoplasma capricolum subsp. capripneumoniae by Loop-mediated isothermal amplification

A set of four specific primers was designed by targeting the H2 gene sequences of Mycoplasma capricolum subsp. capripneumoniae (MCCP). Using Bst DNA polymerase, the products were amplified for 60 min at 65[degrees]C in a simple water bath. Compared with a polymerase chain reaction (PCR) test that ta...

Full description

Saved in:
Bibliographic Details
Published in:African journal of biotechnology 2014-05, Vol.13 (21), p.2113-2118
Main Authors: HE, Ying, ZHANG, Nian-zhang, ZHAO, Ping, CHU, Yue-feng, GAO, Peng-cheng, ZHANG, Jian-jun, LIU, Xiang-tao and, Lu, Zhong-xin
Format: Article
Language:English
Subjects:
Mycoplasma capricolum
Mycoplasma mycoides
Citations: Items that cite this one
Online Access:Get full text
Tags: Add Tag
No Tags, Be the first to tag this record!
cited_by cdi_FETCH-LOGICAL-c1450-f2318c4627e2157f8cc55745cfb8de5ec86273195a23501ffa06788b4e5a49cc3
cites
container_end_page 2118
container_issue 21
container_start_page 2113
container_title African journal of biotechnology
container_volume 13
creator HE, Ying
ZHANG, Nian-zhang
ZHAO, Ping
CHU, Yue-feng
GAO, Peng-cheng
ZHANG, Jian-jun
LIU, Xiang-tao and
Lu, Zhong-xin
description A set of four specific primers was designed by targeting the H2 gene sequences of Mycoplasma capricolum subsp. capripneumoniae (MCCP). Using Bst DNA polymerase, the products were amplified for 60 min at 65[degrees]C in a simple water bath. Compared with a polymerase chain reaction (PCR) test that targets the H2 gene sequences of MCCP, the sensitivity of the loop-mediated isothermal amplification (LAMP) assay was higher (approximately 0.75 fg DNA per reaction). The LAMP products could be visualized by agar gel electrophoresis. There were no cross reactions with other strains in the Mycoplasma mycoides cluster, which indicates the high specificity of the LAMP procedure. The LAMP assay was able to detect MCCP in tissue.
doi_str_mv 10.5897/AJB12.2960
format article
fullrecord <record><control><sourceid>proquest_cross</sourceid><recordid>TN_cdi_proquest_miscellaneous_1654667245</recordid><sourceformat>XML</sourceformat><sourcesystem>PC</sourcesystem><sourcerecordid>1654667245</sourcerecordid><originalsourceid>FETCH-LOGICAL-c1450-f2318c4627e2157f8cc55745cfb8de5ec86273195a23501ffa06788b4e5a49cc3</originalsourceid><addsrcrecordid>eNpNkM1OwzAQhC0EEqVw4Ql8REgptmMnzrFU_KqIA3CONs5aGMWxiRNQ356WcuC0o53RSPMRcs7ZQumqvFo-XnOxEFXBDsiMF1pmKufq8J8-JicpfTAmciHZjHy_YJ_c6L6QQt_SAaJraYsjmtGFngZLnzYmxA6SB2ogDs6EbvI0TU2Ki_0n9jj50DtA2mzoOoSYeWwdjNhSl8L4joOHjoKPnbPOwK75lBxZ6BKe_d05ebu9eV3dZ-vnu4fVcp0ZLhXLrMi5NrIQJQquSquNUaqUythGt6jQ6K2V80qByBXj1gIrSq0biQpkZUw-Jxf73jiEzwnTWHuXDHYd9BimVPNCyaIohVTb6OU-aoaQ0oC23m7zMGxqzuod3fqXbr2jm_8AmsNulw</addsrcrecordid><sourcetype>Aggregation Database</sourcetype><iscdi>true</iscdi><recordtype>article</recordtype><pqid>1654667245</pqid></control><display><type>article</type><title>Sensitive and rapid detection of Mycoplasma capricolum subsp. capripneumoniae by Loop-mediated isothermal amplification</title><source>Free Full-Text Journals in Chemistry</source><creator>HE, Ying ; ZHANG, Nian-zhang ; ZHAO, Ping ; CHU, Yue-feng ; GAO, Peng-cheng ; ZHANG, Jian-jun ; LIU, Xiang-tao and ; Lu, Zhong-xin</creator><creatorcontrib>HE, Ying ; ZHANG, Nian-zhang ; ZHAO, Ping ; CHU, Yue-feng ; GAO, Peng-cheng ; ZHANG, Jian-jun ; LIU, Xiang-tao and ; Lu, Zhong-xin</creatorcontrib><description>A set of four specific primers was designed by targeting the H2 gene sequences of Mycoplasma capricolum subsp. capripneumoniae (MCCP). Using Bst DNA polymerase, the products were amplified for 60 min at 65[degrees]C in a simple water bath. Compared with a polymerase chain reaction (PCR) test that targets the H2 gene sequences of MCCP, the sensitivity of the loop-mediated isothermal amplification (LAMP) assay was higher (approximately 0.75 fg DNA per reaction). The LAMP products could be visualized by agar gel electrophoresis. There were no cross reactions with other strains in the Mycoplasma mycoides cluster, which indicates the high specificity of the LAMP procedure. The LAMP assay was able to detect MCCP in tissue.</description><identifier>ISSN: 1684-5315</identifier><identifier>EISSN: 1684-5315</identifier><identifier>DOI: 10.5897/AJB12.2960</identifier><language>eng</language><subject>Mycoplasma capricolum ; Mycoplasma mycoides</subject><ispartof>African journal of biotechnology, 2014-05, Vol.13 (21), p.2113-2118</ispartof><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c1450-f2318c4627e2157f8cc55745cfb8de5ec86273195a23501ffa06788b4e5a49cc3</citedby></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><link.rule.ids>314,780,784,27924,27925</link.rule.ids></links><search><creatorcontrib>HE, Ying</creatorcontrib><creatorcontrib>ZHANG, Nian-zhang</creatorcontrib><creatorcontrib>ZHAO, Ping</creatorcontrib><creatorcontrib>CHU, Yue-feng</creatorcontrib><creatorcontrib>GAO, Peng-cheng</creatorcontrib><creatorcontrib>ZHANG, Jian-jun</creatorcontrib><creatorcontrib>LIU, Xiang-tao and</creatorcontrib><creatorcontrib>Lu, Zhong-xin</creatorcontrib><title>Sensitive and rapid detection of Mycoplasma capricolum subsp. capripneumoniae by Loop-mediated isothermal amplification</title><title>African journal of biotechnology</title><description>A set of four specific primers was designed by targeting the H2 gene sequences of Mycoplasma capricolum subsp. capripneumoniae (MCCP). Using Bst DNA polymerase, the products were amplified for 60 min at 65[degrees]C in a simple water bath. Compared with a polymerase chain reaction (PCR) test that targets the H2 gene sequences of MCCP, the sensitivity of the loop-mediated isothermal amplification (LAMP) assay was higher (approximately 0.75 fg DNA per reaction). The LAMP products could be visualized by agar gel electrophoresis. There were no cross reactions with other strains in the Mycoplasma mycoides cluster, which indicates the high specificity of the LAMP procedure. The LAMP assay was able to detect MCCP in tissue.</description><subject>Mycoplasma capricolum</subject><subject>Mycoplasma mycoides</subject><issn>1684-5315</issn><issn>1684-5315</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2014</creationdate><recordtype>article</recordtype><recordid>eNpNkM1OwzAQhC0EEqVw4Ql8REgptmMnzrFU_KqIA3CONs5aGMWxiRNQ356WcuC0o53RSPMRcs7ZQumqvFo-XnOxEFXBDsiMF1pmKufq8J8-JicpfTAmciHZjHy_YJ_c6L6QQt_SAaJraYsjmtGFngZLnzYmxA6SB2ogDs6EbvI0TU2Ki_0n9jj50DtA2mzoOoSYeWwdjNhSl8L4joOHjoKPnbPOwK75lBxZ6BKe_d05ebu9eV3dZ-vnu4fVcp0ZLhXLrMi5NrIQJQquSquNUaqUythGt6jQ6K2V80qByBXj1gIrSq0biQpkZUw-Jxf73jiEzwnTWHuXDHYd9BimVPNCyaIohVTb6OU-aoaQ0oC23m7zMGxqzuod3fqXbr2jm_8AmsNulw</recordid><startdate>20140521</startdate><enddate>20140521</enddate><creator>HE, Ying</creator><creator>ZHANG, Nian-zhang</creator><creator>ZHAO, Ping</creator><creator>CHU, Yue-feng</creator><creator>GAO, Peng-cheng</creator><creator>ZHANG, Jian-jun</creator><creator>LIU, Xiang-tao and</creator><creator>Lu, Zhong-xin</creator><scope>AAYXX</scope><scope>CITATION</scope><scope>7QL</scope><scope>7QO</scope><scope>8FD</scope><scope>C1K</scope><scope>FR3</scope><scope>P64</scope></search><sort><creationdate>20140521</creationdate><title>Sensitive and rapid detection of Mycoplasma capricolum subsp. capripneumoniae by Loop-mediated isothermal amplification</title><author>HE, Ying ; ZHANG, Nian-zhang ; ZHAO, Ping ; CHU, Yue-feng ; GAO, Peng-cheng ; ZHANG, Jian-jun ; LIU, Xiang-tao and ; Lu, Zhong-xin</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c1450-f2318c4627e2157f8cc55745cfb8de5ec86273195a23501ffa06788b4e5a49cc3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2014</creationdate><topic>Mycoplasma capricolum</topic><topic>Mycoplasma mycoides</topic><toplevel>online_resources</toplevel><creatorcontrib>HE, Ying</creatorcontrib><creatorcontrib>ZHANG, Nian-zhang</creatorcontrib><creatorcontrib>ZHAO, Ping</creatorcontrib><creatorcontrib>CHU, Yue-feng</creatorcontrib><creatorcontrib>GAO, Peng-cheng</creatorcontrib><creatorcontrib>ZHANG, Jian-jun</creatorcontrib><creatorcontrib>LIU, Xiang-tao and</creatorcontrib><creatorcontrib>Lu, Zhong-xin</creatorcontrib><collection>CrossRef</collection><collection>Bacteriology Abstracts (Microbiology B)</collection><collection>Biotechnology Research Abstracts</collection><collection>Technology Research Database</collection><collection>Environmental Sciences and Pollution Management</collection><collection>Engineering Research Database</collection><collection>Biotechnology and BioEngineering Abstracts</collection><jtitle>African journal of biotechnology</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>HE, Ying</au><au>ZHANG, Nian-zhang</au><au>ZHAO, Ping</au><au>CHU, Yue-feng</au><au>GAO, Peng-cheng</au><au>ZHANG, Jian-jun</au><au>LIU, Xiang-tao and</au><au>Lu, Zhong-xin</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Sensitive and rapid detection of Mycoplasma capricolum subsp. capripneumoniae by Loop-mediated isothermal amplification</atitle><jtitle>African journal of biotechnology</jtitle><date>2014-05-21</date><risdate>2014</risdate><volume>13</volume><issue>21</issue><spage>2113</spage><epage>2118</epage><pages>2113-2118</pages><issn>1684-5315</issn><eissn>1684-5315</eissn><abstract>A set of four specific primers was designed by targeting the H2 gene sequences of Mycoplasma capricolum subsp. capripneumoniae (MCCP). Using Bst DNA polymerase, the products were amplified for 60 min at 65[degrees]C in a simple water bath. Compared with a polymerase chain reaction (PCR) test that targets the H2 gene sequences of MCCP, the sensitivity of the loop-mediated isothermal amplification (LAMP) assay was higher (approximately 0.75 fg DNA per reaction). The LAMP products could be visualized by agar gel electrophoresis. There were no cross reactions with other strains in the Mycoplasma mycoides cluster, which indicates the high specificity of the LAMP procedure. The LAMP assay was able to detect MCCP in tissue.</abstract><doi>10.5897/AJB12.2960</doi><tpages>6</tpages><oa>free_for_read</oa></addata></record>
fulltext fulltext
identifier ISSN: 1684-5315
ispartof African journal of biotechnology, 2014-05, Vol.13 (21), p.2113-2118
issn 1684-5315
1684-5315
language eng
recordid cdi_proquest_miscellaneous_1654667245
source Free Full-Text Journals in Chemistry
subjects Mycoplasma capricolum
Mycoplasma mycoides
title Sensitive and rapid detection of Mycoplasma capricolum subsp. capripneumoniae by Loop-mediated isothermal amplification
url http://sfxeu10.hosted.exlibrisgroup.com/loughborough?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&ctx_tim=2025-01-01T03%3A17%3A52IST&url_ver=Z39.88-2004&url_ctx_fmt=infofi/fmt:kev:mtx:ctx&rfr_id=info:sid/primo.exlibrisgroup.com:primo3-Article-proquest_cross&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.atitle=Sensitive%20and%20rapid%20detection%20of%20Mycoplasma%20capricolum%20subsp.%20capripneumoniae%20by%20Loop-mediated%20isothermal%20amplification&rft.jtitle=African%20journal%20of%20biotechnology&rft.au=HE,%20Ying&rft.date=2014-05-21&rft.volume=13&rft.issue=21&rft.spage=2113&rft.epage=2118&rft.pages=2113-2118&rft.issn=1684-5315&rft.eissn=1684-5315&rft_id=info:doi/10.5897/AJB12.2960&rft_dat=%3Cproquest_cross%3E1654667245%3C/proquest_cross%3E%3Cgrp_id%3Ecdi_FETCH-LOGICAL-c1450-f2318c4627e2157f8cc55745cfb8de5ec86273195a23501ffa06788b4e5a49cc3%3C/grp_id%3E%3Coa%3E%3C/oa%3E%3Curl%3E%3C/url%3E&rft_id=info:oai/&rft_pqid=1654667245&rft_id=info:pmid/&rfr_iscdi=true