DNAzyme-based biosensors and nanodevices

DNAzymes, screened through in vitro selection, have shown great promise as molecular tools in the design of biosensors and nanodevices. The catalytic activities of DNAzymes depend specifically on cofactors and show multiple enzymatic turnover properties, which make DNAzymes both versatile recognitio...

Full description

Saved in:
Bibliographic Details
Published in:Chemical communications (Cambridge, England) England), 2015-01, Vol.51 (6), p.979-995
Main Authors: Gong, Liang, Zhao, Zilong, Lv, Yi-Fan, Huan, Shuang-Yan, Fu, Ting, Zhang, Xiao-Bing, Shen, Guo-Li, Yu, Ru-Qin
Format: Article
Language:English
Subjects:
Amplifiers
Biosensing Techniques
Biosensors
Complement
Deoxyribonucleic acid
DNA, Catalytic - metabolism
Nanostructure
Nanotechnology
Nanotechnology devices
Raman scattering
Ribonucleic acids
Citations: Items that this one cites
Items that cite this one
Online Access:Get full text
Tags: Add Tag
No Tags, Be the first to tag this record!
Description
Summary:DNAzymes, screened through in vitro selection, have shown great promise as molecular tools in the design of biosensors and nanodevices. The catalytic activities of DNAzymes depend specifically on cofactors and show multiple enzymatic turnover properties, which make DNAzymes both versatile recognition elements and outstanding signal amplifiers. Combining nanomaterials with unique optical, magnetic and electronic properties, DNAzymes may yield novel fluorescent, colorimetric, surface-enhanced Raman scattering (SERS), electrochemical and chemiluminescent biosensors. Moreover, some DNAzymes have been utilized as functional components to perform arithmetic operations or as "walkers" to move along DNA tracks. DNAzymes can also function as promising therapeutics, when designed to complement target mRNAs or viral RNAs, and consequently lead to down-regulation of protein expression. This feature article focuses on the most significant achievements in using DNAzymes as recognition elements and signal amplifiers for biosensors, and highlights the applications of DNAzymes in logic gates, DNA walkers and nanotherapeutics. DNAzymes, screened through in vitro selection, have shown great promise as molecular tools in the design of biosensors and nanodevices.
ISSN:1359-7345
1364-548X
DOI:10.1039/c4cc06855f