Glutamate sol-gel amperometric biosensor based on co-immobilised NADP+ and glutamate dehydrogenase

In this work the preparation and electrochemical characterisation of a new glutamate amperometric sol-gel biosensor is described. A carbon paste electrode was electrochemically modified with the phenothiazine dye methylene green ( MG ). The NADP + and glutamate dehydrogenase were co-immobilised in a...

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Bibliographic Details
Published in:Journal of analytical chemistry (New York, N.Y.) N.Y.), 2013-09, Vol.68 (9), p.794-800
Main Authors: Gomes, S. P., Doležalová, J., Araújo, A. N., Couto, C. M. C. M., Montenegro, M. C. B. S. M.
Format: Article
Language:English
Subjects:
Analytical Chemistry
Chemistry
Chemistry and Materials Science
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Summary:In this work the preparation and electrochemical characterisation of a new glutamate amperometric sol-gel biosensor is described. A carbon paste electrode was electrochemically modified with the phenothiazine dye methylene green ( MG ). The NADP + and glutamate dehydrogenase were co-immobilised in a sol-gel matrix. When coupled to a flow injection system ( FIA ) the biosensor showed good electrocatalytic activity towards NADPH oxidation at the potential of + 0.3 V vs. Ag/AgCl; KCl sat., which represents a strong overpotential reduction. The biosensor yielded a linear response from 5.0 × 10 −5 to 1.0 × 10 −2 M glutamate concentration, with a detection limit of 5 × 10 −6 M and reproducibility of results better than 2.3% ( RSD ). Moreover, the implemented biosensor showed to be still useful after 7 months, 500 determinations.
ISSN:1061-9348
1608-3199
DOI:10.1134/S1061934813090049