Global Characterization of Differential Gene Expression Profiles in Mouse V gamma 1+ and V gamma 4+ gamma delta T Cells: e112964

• Published in: PloS one 2014-11, Vol.9 (11)
• Main Authors: Dong, Peng, Zhang, Siya, Cai, Menghua, Kang, Ning, Hu, Yu, Cui, Lianxian, Zhang, Jianmin, He, Wei
• Format: Article
• Language: English
• ISSN: 1932-6203
• DOI: 10.1371/journal.pone.0112964

Peripheral gamma delta T cells in mice are classified into two major subpopulations, V gamma 1+ and V gamma 4+, based on the composition of T cell receptors. However, their intrinsic differences remain unclear. In this study, we analyzed gene expression profiles of the two subsets using Illumina HiSeq 2000 Sequencer. We identified 1995 transcripts related to the activation of V gamma 1+ gamma delta T cells, and 2158 transcripts related to the activation of V gamma 4+ gamma delta T cells. We identified 24 transcripts differentially expressed between the two subsets in resting condition, and 20 after PMA/Ionomycin treatment. We found that both cell types maintained phenotypes producing IFN- gamma , TNF- alpha , TGF- beta and IL-10. However, V gamma 1+ gamma delta T cells produced more Th2 type cytokines, such as IL-4 and IL-5, while V gamma 4+ gamma delta T cells preferentially produced IL-17. Our study provides a comprehensive gene expression profile of mouse peripheral V gamma 1+ and V gamma 4+ gamma delta T cells that describes the inherent differences between them.