Isolation of crustacean hyperglycemic hormone-family peptides with vitellogenesis-inhibiting activity from the shiba shrimp Metapenaeus joyneri

Vitellogenesis-inhibiting hormone (VIH) in Crustacea belongs to the crustacean hyperglycemic hormone (CHH)-family. In order to characterize multiple VIH molecules in the shiba shrimp Metapenaeus joyneri , four CHH-family peptides designated as Mej-SGP-I, -II, -III, and -IV were purified by reversed-...

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Bibliographic Details
Published in:Fisheries science 2015-01, Vol.81 (1), p.65-72
Main Authors: Fukushima, Midori, Kotaka, Sayaka, Tsutsui, Naoaki, Asahina, Kiyoshi, Izumi, Susumu, Ohira, Tsuyoshi
Format: Article
Language:English
Subjects:
Amino acids
Aquaculture
Biomedical and Life Sciences
Crustacea
Crustaceans
Fish & Wildlife Biology & Management
Fisheries
Fishing
Food Science
Freshwater & Marine Ecology
Gene expression
Hormones
Life Sciences
Liquid chromatography
Litopenaeus vannamei
Marine
Marsupenaeus japonicus
Metapenaeus joyneri
Neuropeptides
Original Article
Ovaries
Peptides
Selective breeding
Shellfish
Sinuses
Studies
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Summary:Vitellogenesis-inhibiting hormone (VIH) in Crustacea belongs to the crustacean hyperglycemic hormone (CHH)-family. In order to characterize multiple VIH molecules in the shiba shrimp Metapenaeus joyneri , four CHH-family peptides designated as Mej-SGP-I, -II, -III, and -IV were purified by reversed-phase HPLC and identified by N-terminal amino acid sequencing. The vitellogenesis-inhibiting activities of these peptides were examined using ex vivo incubation of ovarian fragments of the kuruma prawn Marsupenaeus japonicus . All of the four peptides significantly reduced vitellogenin mRNA levels in the ovarian fragments. Among them, the inhibitory activity of Mej-SGP-III which was the most abundant CHH-family peptide in the sinus gland was apparent. Therefore, a cDNA encoding the Mej-SGP-III precursor was cloned by RT-PCR coupled with 5′- and 3′-RACE. The mature Mej-SGP-III was found to consist of 72 amino acid residues containing six conserved cysteine residues and possess an amidated C-terminus. The mature Mej-SGP-III showed the highest sequence identity to Pej-SGP-V (one of six CHH/VIHs in M. japonicus ) and Liv-SGP-G (one of six VIHs in Litopenaeus vannamei ). These results indicate that Mej-SGP-III is one of the hormones regulating vitellogenesis in M. joyneri .
ISSN:0919-9268
1444-2906
DOI:10.1007/s12562-014-0834-7