Isolation and Expression of Enolase Gene in Fusarium oxysporum f. sp. lycopersici

Fusarium oxysporum f. sp. lycopersici is a fungus responsible for the tomato disease known as fusariosis. Enolase, which is the enzyme that catalyzes the reaction of 2-phosphoglycerate to phosphoenolpyruvate, is present during glycolysis. Enolase genes have been isolated from bacteria and fungi, amo...

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Bibliographic Details
Published in:Applied biochemistry and biotechnology 2015-01, Vol.175 (2), p.902-908
Main Authors: Macías-Sánchez, Karla Lizbeth, García-Soto, Jesús, Roncero, M. Isabel G, Hernández-Monjaraz, Wendy, Caudillo-Pérez, César, Martínez-Cadena, Ma. Guadalupe
Format: Article
Language:English
Subjects:
Amino Acid Sequence
Aspergillus oryzae
bacteria
Biochemistry
Biotechnology
Candida albicans
carbon
Carbon sources
Chemistry
Chemistry and Materials Science
Crop diseases
Enzymes
Fungi
Fusarium - enzymology
Fusarium - genetics
Fusarium - growth & development
Fusarium oxysporum
Fusarium oxysporum f. sp. lycopersici
Gene Expression
genes
Glucose
glycolysis
Growth conditions
Lycopersicon esculentum
Microorganisms
Molecular Sequence Data
nucleotide sequences
phosphopyruvate hydratase
Phosphopyruvate Hydratase - chemistry
Phosphopyruvate Hydratase - genetics
Phosphopyruvate Hydratase - isolation & purification
reverse transcriptase polymerase chain reaction
Tomatoes
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Summary:Fusarium oxysporum f. sp. lycopersici is a fungus responsible for the tomato disease known as fusariosis. Enolase, which is the enzyme that catalyzes the reaction of 2-phosphoglycerate to phosphoenolpyruvate, is present during glycolysis. Enolase genes have been isolated from bacteria and fungi, among other organisms. In this research, a large portion of the enolase, eno, gene sequence was isolated from F. oxysporum and compared with those of other microorganisms, revealing a similarity of 51–69 %. We analyzed the copy number of the eno gene and determined that only a single copy is present in F. oxysporum, as in several fungi, such as Candida albicans and Aspergillus oryzae. We also detected the expression of the eno gene by reverse transcription-polymerase chain reaction during in vitro growth under two growth conditions where glucose was used as the carbon source, and we observed the same eno gene expression levels under both growth conditions.
ISSN:0273-2289
1559-0291
DOI:10.1007/s12010-014-1338-8