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Effect of Inoculation Process on Lycopene Production by Blakeslea trispora in a Stirred-Tank Reactor
Lycopene biosynthesis by Blakeslea trispora was greatly enhanced in a stirred-tank reactor when a nonsynchronous inoculation process, in which the (+) mating type was inoculated after the (−) mating type has been grown for a certain period of time, was applied. The lycopene concentration with nonsyn...
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| Published in: | Applied biochemistry and biotechnology 2015-01, Vol.175 (2), p.770-779 |
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| cites | cdi_FETCH-LOGICAL-c466t-5e87ebd509bcb7b9980c43e4bbde2c9419def40c5d141d8508d1aed2ba9c54bc3 |
| container_end_page | 779 |
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| container_title | Applied biochemistry and biotechnology |
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| creator | Wang, Qiang Feng, Ling-ran Luo, Wei Li, Han-guang Zhou, Ya Yu, Xiao-bin |
| description | Lycopene biosynthesis by Blakeslea trispora was greatly enhanced in a stirred-tank reactor when a nonsynchronous inoculation process, in which the (+) mating type was inoculated after the (−) mating type has been grown for a certain period of time, was applied. The lycopene concentration with nonsynchronous inoculation in a 24-h inoculation interval was 33 % higher than that with synchronous inoculation. The optimum inoculation ratio was 1:2 (+/−) at the 36 and 48 h inoculum age of mating types (+) and (−), respectively. Fermentation time for the individual strains and mated conditions showed that the (+) mating type grows faster than the (−) mating type. Morphological observation showed that the mycelium ratio of B. trispora (−) in mating culture with nonsynchronous inoculation was higher than that with synchronous inoculation. The results indicated that nonsynchronous inoculation process increased the dominance of B. trispora (−) in joint cultivation and hence stimulated lycopene biosynthesis. |
| doi_str_mv | 10.1007/s12010-014-1327-y |
| format | article |
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The lycopene concentration with nonsynchronous inoculation in a 24-h inoculation interval was 33 % higher than that with synchronous inoculation. The optimum inoculation ratio was 1:2 (+/−) at the 36 and 48 h inoculum age of mating types (+) and (−), respectively. Fermentation time for the individual strains and mated conditions showed that the (+) mating type grows faster than the (−) mating type. Morphological observation showed that the mycelium ratio of B. trispora (−) in mating culture with nonsynchronous inoculation was higher than that with synchronous inoculation. The results indicated that nonsynchronous inoculation process increased the dominance of B. trispora (−) in joint cultivation and hence stimulated lycopene biosynthesis.</description><identifier>ISSN: 0273-2289</identifier><identifier>EISSN: 1559-0291</identifier><identifier>DOI: 10.1007/s12010-014-1327-y</identifier><identifier>PMID: 25342268</identifier><language>eng</language><publisher>Boston: Springer-Verlag</publisher><subject>Antioxidants ; Biochemistry ; Biomass ; Bioreactors ; Biosynthesis ; Biotechnology ; Blakeslea trispora ; Carotenoids - biosynthesis ; Chemistry ; Chemistry and Materials Science ; Fermentation ; Glucose - metabolism ; Hydrogen-Ion Concentration ; inoculum ; lycopene ; Mating Factor ; mating types ; Mucorales - metabolism ; mycelium ; Mycelium - metabolism ; Peptides - physiology ; Phytochemicals ; Pigments ; Reactors ; Time Factors</subject><ispartof>Applied biochemistry and biotechnology, 2015-01, Vol.175 (2), p.770-779</ispartof><rights>Springer Science+Business Media New York 2014</rights><rights>Springer Science+Business Media New York 2015</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c466t-5e87ebd509bcb7b9980c43e4bbde2c9419def40c5d141d8508d1aed2ba9c54bc3</citedby><cites>FETCH-LOGICAL-c466t-5e87ebd509bcb7b9980c43e4bbde2c9419def40c5d141d8508d1aed2ba9c54bc3</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><link.rule.ids>314,780,784,27924,27925</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/25342268$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Wang, Qiang</creatorcontrib><creatorcontrib>Feng, Ling-ran</creatorcontrib><creatorcontrib>Luo, Wei</creatorcontrib><creatorcontrib>Li, Han-guang</creatorcontrib><creatorcontrib>Zhou, Ya</creatorcontrib><creatorcontrib>Yu, Xiao-bin</creatorcontrib><title>Effect of Inoculation Process on Lycopene Production by Blakeslea trispora in a Stirred-Tank Reactor</title><title>Applied biochemistry and biotechnology</title><addtitle>Appl Biochem Biotechnol</addtitle><addtitle>Appl Biochem Biotechnol</addtitle><description>Lycopene biosynthesis by Blakeslea trispora was greatly enhanced in a stirred-tank reactor when a nonsynchronous inoculation process, in which the (+) mating type was inoculated after the (−) mating type has been grown for a certain period of time, was applied. The lycopene concentration with nonsynchronous inoculation in a 24-h inoculation interval was 33 % higher than that with synchronous inoculation. The optimum inoculation ratio was 1:2 (+/−) at the 36 and 48 h inoculum age of mating types (+) and (−), respectively. Fermentation time for the individual strains and mated conditions showed that the (+) mating type grows faster than the (−) mating type. Morphological observation showed that the mycelium ratio of B. trispora (−) in mating culture with nonsynchronous inoculation was higher than that with synchronous inoculation. The results indicated that nonsynchronous inoculation process increased the dominance of B. trispora (−) in joint cultivation and hence stimulated lycopene biosynthesis.</description><subject>Antioxidants</subject><subject>Biochemistry</subject><subject>Biomass</subject><subject>Bioreactors</subject><subject>Biosynthesis</subject><subject>Biotechnology</subject><subject>Blakeslea trispora</subject><subject>Carotenoids - biosynthesis</subject><subject>Chemistry</subject><subject>Chemistry and Materials Science</subject><subject>Fermentation</subject><subject>Glucose - metabolism</subject><subject>Hydrogen-Ion Concentration</subject><subject>inoculum</subject><subject>lycopene</subject><subject>Mating Factor</subject><subject>mating types</subject><subject>Mucorales - metabolism</subject><subject>mycelium</subject><subject>Mycelium - metabolism</subject><subject>Peptides - physiology</subject><subject>Phytochemicals</subject><subject>Pigments</subject><subject>Reactors</subject><subject>Time Factors</subject><issn>0273-2289</issn><issn>1559-0291</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2015</creationdate><recordtype>article</recordtype><recordid>eNqNkc1u1DAURi1ERYfCA7ABS2zYBHwd24mXtCpQaaRWtF1b_rmp0mbiwU4WeXs8TUGIBWJly_d835V1CHkD7CMw1nzKwBmwioGooOZNtTwjG5BSV4xreE42jDd1xXmrj8nLnO8ZA97K5gU55rIWnKt2Q8J516GfaOzoxRj9PNipjyO9StFjzrRct4uPexzx8BZm_zh2Cz0d7APmAS2dUp_3MVnaj9TS66lPCUN1Y8cH-h2tn2J6RY46O2R8_XSekNsv5zdn36rt5deLs8_bygulpkpi26ALkmnnXeO0bpkXNQrnAnKvBeiAnWBeBhAQWsnaABYDd1Z7KZyvT8iHtXef4o8Z82R2ffY4DHbEOGcDSgrV1kry_0F5rWvFVEHf_4XexzmN5SOFEgqg5QoKBSvlU8w5YWf2qd_ZtBhg5mDLrLZMsWUOtsxSMm-fmme3w_A78UtPAfgK5DIa7zD9sfofre_WUGejsXfFjrm9LpAs_pVSAuqfzF6pBg</recordid><startdate>20150101</startdate><enddate>20150101</enddate><creator>Wang, Qiang</creator><creator>Feng, Ling-ran</creator><creator>Luo, Wei</creator><creator>Li, Han-guang</creator><creator>Zhou, Ya</creator><creator>Yu, Xiao-bin</creator><general>Springer-Verlag</general><general>Springer US</general><general>Springer Nature B.V</general><scope>FBQ</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>3V.</scope><scope>7ST</scope><scope>7T7</scope><scope>7TM</scope><scope>7X7</scope><scope>7XB</scope><scope>88A</scope><scope>88E</scope><scope>88I</scope><scope>8AO</scope><scope>8FD</scope><scope>8FE</scope><scope>8FH</scope><scope>8FI</scope><scope>8FJ</scope><scope>8FK</scope><scope>ABUWG</scope><scope>AFKRA</scope><scope>AZQEC</scope><scope>BBNVY</scope><scope>BENPR</scope><scope>BHPHI</scope><scope>C1K</scope><scope>CCPQU</scope><scope>DWQXO</scope><scope>FR3</scope><scope>FYUFA</scope><scope>GHDGH</scope><scope>GNUQQ</scope><scope>HCIFZ</scope><scope>K9.</scope><scope>LK8</scope><scope>M0S</scope><scope>M1P</scope><scope>M2P</scope><scope>M7P</scope><scope>P64</scope><scope>PQEST</scope><scope>PQQKQ</scope><scope>PQUKI</scope><scope>Q9U</scope><scope>RC3</scope><scope>SOI</scope><scope>7X8</scope><scope>7QO</scope></search><sort><creationdate>20150101</creationdate><title>Effect of Inoculation Process on Lycopene Production by Blakeslea trispora in a Stirred-Tank Reactor</title><author>Wang, Qiang ; Feng, Ling-ran ; Luo, Wei ; Li, Han-guang ; Zhou, Ya ; Yu, Xiao-bin</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c466t-5e87ebd509bcb7b9980c43e4bbde2c9419def40c5d141d8508d1aed2ba9c54bc3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2015</creationdate><topic>Antioxidants</topic><topic>Biochemistry</topic><topic>Biomass</topic><topic>Bioreactors</topic><topic>Biosynthesis</topic><topic>Biotechnology</topic><topic>Blakeslea trispora</topic><topic>Carotenoids - biosynthesis</topic><topic>Chemistry</topic><topic>Chemistry and Materials Science</topic><topic>Fermentation</topic><topic>Glucose - metabolism</topic><topic>Hydrogen-Ion Concentration</topic><topic>inoculum</topic><topic>lycopene</topic><topic>Mating Factor</topic><topic>mating types</topic><topic>Mucorales - metabolism</topic><topic>mycelium</topic><topic>Mycelium - metabolism</topic><topic>Peptides - physiology</topic><topic>Phytochemicals</topic><topic>Pigments</topic><topic>Reactors</topic><topic>Time Factors</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Wang, Qiang</creatorcontrib><creatorcontrib>Feng, Ling-ran</creatorcontrib><creatorcontrib>Luo, Wei</creatorcontrib><creatorcontrib>Li, Han-guang</creatorcontrib><creatorcontrib>Zhou, Ya</creatorcontrib><creatorcontrib>Yu, Xiao-bin</creatorcontrib><collection>AGRIS</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>ProQuest Central (Corporate)</collection><collection>Environment Abstracts</collection><collection>Industrial and Applied Microbiology Abstracts (Microbiology A)</collection><collection>Nucleic Acids Abstracts</collection><collection>ProQuest Health & Medical Collection</collection><collection>ProQuest Central (purchase pre-March 2016)</collection><collection>Biology Database (Alumni Edition)</collection><collection>Medical Database (Alumni Edition)</collection><collection>Science Database (Alumni Edition)</collection><collection>ProQuest Pharma Collection</collection><collection>Technology Research Database</collection><collection>ProQuest SciTech Collection</collection><collection>ProQuest Natural Science Collection</collection><collection>Hospital Premium Collection</collection><collection>Hospital Premium Collection (Alumni Edition)</collection><collection>ProQuest Central (Alumni) (purchase pre-March 2016)</collection><collection>ProQuest Central (Alumni)</collection><collection>ProQuest Central</collection><collection>ProQuest Central Essentials</collection><collection>Biological Science Collection</collection><collection>AUTh Library subscriptions: ProQuest Central</collection><collection>ProQuest Natural Science Collection</collection><collection>Environmental Sciences and Pollution Management</collection><collection>ProQuest One Community College</collection><collection>ProQuest Central</collection><collection>Engineering Research Database</collection><collection>Health Research Premium Collection</collection><collection>Health Research Premium Collection (Alumni)</collection><collection>ProQuest Central Student</collection><collection>SciTech Premium Collection</collection><collection>ProQuest Health & Medical Complete (Alumni)</collection><collection>ProQuest Biological Science Collection</collection><collection>Health & Medical Collection (Alumni Edition)</collection><collection>PML(ProQuest Medical Library)</collection><collection>ProQuest Science Journals</collection><collection>Biological Science Database</collection><collection>Biotechnology and BioEngineering Abstracts</collection><collection>ProQuest One Academic Eastern Edition (DO NOT USE)</collection><collection>ProQuest One Academic</collection><collection>ProQuest One Academic UKI Edition</collection><collection>ProQuest Central Basic</collection><collection>Genetics Abstracts</collection><collection>Environment Abstracts</collection><collection>MEDLINE - Academic</collection><collection>Biotechnology Research Abstracts</collection><jtitle>Applied biochemistry and biotechnology</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Wang, Qiang</au><au>Feng, Ling-ran</au><au>Luo, Wei</au><au>Li, Han-guang</au><au>Zhou, Ya</au><au>Yu, Xiao-bin</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Effect of Inoculation Process on Lycopene Production by Blakeslea trispora in a Stirred-Tank Reactor</atitle><jtitle>Applied biochemistry and biotechnology</jtitle><stitle>Appl Biochem Biotechnol</stitle><addtitle>Appl Biochem Biotechnol</addtitle><date>2015-01-01</date><risdate>2015</risdate><volume>175</volume><issue>2</issue><spage>770</spage><epage>779</epage><pages>770-779</pages><issn>0273-2289</issn><eissn>1559-0291</eissn><abstract>Lycopene biosynthesis by Blakeslea trispora was greatly enhanced in a stirred-tank reactor when a nonsynchronous inoculation process, in which the (+) mating type was inoculated after the (−) mating type has been grown for a certain period of time, was applied. The lycopene concentration with nonsynchronous inoculation in a 24-h inoculation interval was 33 % higher than that with synchronous inoculation. The optimum inoculation ratio was 1:2 (+/−) at the 36 and 48 h inoculum age of mating types (+) and (−), respectively. Fermentation time for the individual strains and mated conditions showed that the (+) mating type grows faster than the (−) mating type. Morphological observation showed that the mycelium ratio of B. trispora (−) in mating culture with nonsynchronous inoculation was higher than that with synchronous inoculation. The results indicated that nonsynchronous inoculation process increased the dominance of B. trispora (−) in joint cultivation and hence stimulated lycopene biosynthesis.</abstract><cop>Boston</cop><pub>Springer-Verlag</pub><pmid>25342268</pmid><doi>10.1007/s12010-014-1327-y</doi><tpages>10</tpages></addata></record> |
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| subjects | Antioxidants Biochemistry Biomass Bioreactors Biosynthesis Biotechnology Blakeslea trispora Carotenoids - biosynthesis Chemistry Chemistry and Materials Science Fermentation Glucose - metabolism Hydrogen-Ion Concentration inoculum lycopene Mating Factor mating types Mucorales - metabolism mycelium Mycelium - metabolism Peptides - physiology Phytochemicals Pigments Reactors Time Factors |
| title | Effect of Inoculation Process on Lycopene Production by Blakeslea trispora in a Stirred-Tank Reactor |
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