Assessment of Phaseolus vulgaris L and Vigna unguiculata (L.) Walp leaves for antifungal metabolites against two bean fungal pathogens Colletotricum lindemuthianum and Phaeoisariopsis griseola

The antifungal potential of hydro-ethanolic leaf extracts of bean varieties was analyzed by bioautography, assessing the contribution of defense molecules of proteic nature against two bean fungal pathogens Colletotricum lindemuthianum and Phaeoisariopsis griseola, also, the Rf values and relative a...

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Bibliographic Details
Published in:African journal of biotechnology 2014-04, Vol.13 (15), p.1657-1665
Main Authors: Diana, Alex, ra, Norentilde, a-Ramirez, Oscar, Julian Velasquez-Ballesteros, Elizabeth, Murillo-Perea, Jonh, Jairo Mendez-Arteaga, Jose, Fern, o, Solanilla-Duque, Walter, Murillo-Arango
Format: Article
Language:English
Subjects:
Phaeoisariopsis griseola
Phaseolus vulgaris
Vigna
Vigna unguiculata
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Summary:The antifungal potential of hydro-ethanolic leaf extracts of bean varieties was analyzed by bioautography, assessing the contribution of defense molecules of proteic nature against two bean fungal pathogens Colletotricum lindemuthianum and Phaeoisariopsis griseola, also, the Rf values and relative activities of separated compounds were determined; these compounds were in a range of medium polarity to high polarity. The bioactivity expressed by the studied bean varieties could be correlated with the presence of proteic nature compounds, in joint action with secondary plant metabolites. There was some similarity in the chemical composition of the components of the extracts. The varieties G18350, G14241, G1320 CIAT and Vigna ungiculata were the most promising for isolating antifungal compounds. The results demonstrate the value of bioautography as a simple and cheap method to examine plant extracts with antifungal activity.
ISSN:1684-5315
1684-5315
DOI:10.5897/AJB2013.12810