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Expression of dnaK and groESL operons during sporulation of Bacillus megaterium
Abstract Expression of the dnaK and groEL genes during sporulation was assayed by determination of their mRNA levels by Northern blotting and compared with the relative level and rate of synthesis of the corresponding proteins. The ability of sporulating cells to respond to a heat shock by an increa...
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| Published in: | FEMS microbiology letters 1998-08, Vol.165 (1), p.181-186 |
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| Main Authors: | , , , , , , , |
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| container_end_page | 186 |
| container_issue | 1 |
| container_start_page | 181 |
| container_title | FEMS microbiology letters |
| container_volume | 165 |
| creator | Hlaváček, Otakar Adamec, Jiřı́ Vomastek, Tomáš Babková, Lenka Sedlák, Miroslav Vohradský, Jiřı́ Váchová, Libuše Chaloupka, Jiřı́ |
| description | Abstract
Expression of the dnaK and groEL genes during sporulation was assayed by determination of their mRNA levels by Northern blotting and compared with the relative level and rate of synthesis of the corresponding proteins. The ability of sporulating cells to respond to a heat shock by an increase in dnaK and groEL expression was determined at the same time. Synthesis of DnaK and GroEL encoding mRNAs during sporulation in non-shocked cells was low suggesting that this kind of cytodifferentiation was not accompanied by enhanced synthesis of these chaperones. Also the ability of sporulating cells to respond to a heat shock by stimulating their synthesis substantially decreased during the reversible and dropped to negligible values during the irreversible sporulation phase. Nevertheless, some dependence of the heat shock response on sporulation exists because sporulation suppression by mutation or by netropsin treatment further decreased the cells' capacity to respond to a heat shock. |
| doi_str_mv | 10.1111/j.1574-6968.1998.tb13144.x |
| format | article |
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Expression of the dnaK and groEL genes during sporulation was assayed by determination of their mRNA levels by Northern blotting and compared with the relative level and rate of synthesis of the corresponding proteins. The ability of sporulating cells to respond to a heat shock by an increase in dnaK and groEL expression was determined at the same time. Synthesis of DnaK and GroEL encoding mRNAs during sporulation in non-shocked cells was low suggesting that this kind of cytodifferentiation was not accompanied by enhanced synthesis of these chaperones. Also the ability of sporulating cells to respond to a heat shock by stimulating their synthesis substantially decreased during the reversible and dropped to negligible values during the irreversible sporulation phase. Nevertheless, some dependence of the heat shock response on sporulation exists because sporulation suppression by mutation or by netropsin treatment further decreased the cells' capacity to respond to a heat shock.</description><identifier>ISSN: 0378-1097</identifier><identifier>EISSN: 1574-6968</identifier><identifier>DOI: 10.1111/j.1574-6968.1998.tb13144.x</identifier><identifier>CODEN: FMLED7</identifier><language>eng</language><publisher>Oxford, UK: Blackwell Publishing Ltd</publisher><subject>Bacillus megaterium ; Bacteriology ; Biological and medical sciences ; Chaperones ; DnaK protein ; Fundamental and applied biological sciences. Psychology ; Gene expression ; Genetics ; Heat shock ; Heat shock protein ; Microbiology ; mRNA ; Mutation ; Northern blotting ; Operons ; Sporulation ; Synthesis</subject><ispartof>FEMS microbiology letters, 1998-08, Vol.165 (1), p.181-186</ispartof><rights>1998 Federation of European Microbiological Societies. Published by Elsevier Science B.V. All rights reserved 1998</rights><rights>1998 INIST-CNRS</rights><rights>1998 Federation of European Microbiological Societies. Published by Elsevier Science B.V. All rights reserved</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><cites>FETCH-LOGICAL-c3711-9d4fd184cc48bc0acd45e77a1a50b3b41798aac21e1affec7bc0af50a41e36a13</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><link.rule.ids>314,780,784,27924,27925</link.rule.ids><backlink>$$Uhttp://pascal-francis.inist.fr/vibad/index.php?action=getRecordDetail&idt=2375816$$DView record in Pascal Francis$$Hfree_for_read</backlink></links><search><creatorcontrib>Hlaváček, Otakar</creatorcontrib><creatorcontrib>Adamec, Jiřı́</creatorcontrib><creatorcontrib>Vomastek, Tomáš</creatorcontrib><creatorcontrib>Babková, Lenka</creatorcontrib><creatorcontrib>Sedlák, Miroslav</creatorcontrib><creatorcontrib>Vohradský, Jiřı́</creatorcontrib><creatorcontrib>Váchová, Libuše</creatorcontrib><creatorcontrib>Chaloupka, Jiřı́</creatorcontrib><title>Expression of dnaK and groESL operons during sporulation of Bacillus megaterium</title><title>FEMS microbiology letters</title><description>Abstract
Expression of the dnaK and groEL genes during sporulation was assayed by determination of their mRNA levels by Northern blotting and compared with the relative level and rate of synthesis of the corresponding proteins. The ability of sporulating cells to respond to a heat shock by an increase in dnaK and groEL expression was determined at the same time. Synthesis of DnaK and GroEL encoding mRNAs during sporulation in non-shocked cells was low suggesting that this kind of cytodifferentiation was not accompanied by enhanced synthesis of these chaperones. Also the ability of sporulating cells to respond to a heat shock by stimulating their synthesis substantially decreased during the reversible and dropped to negligible values during the irreversible sporulation phase. Nevertheless, some dependence of the heat shock response on sporulation exists because sporulation suppression by mutation or by netropsin treatment further decreased the cells' capacity to respond to a heat shock.</description><subject>Bacillus megaterium</subject><subject>Bacteriology</subject><subject>Biological and medical sciences</subject><subject>Chaperones</subject><subject>DnaK protein</subject><subject>Fundamental and applied biological sciences. Psychology</subject><subject>Gene expression</subject><subject>Genetics</subject><subject>Heat shock</subject><subject>Heat shock protein</subject><subject>Microbiology</subject><subject>mRNA</subject><subject>Mutation</subject><subject>Northern blotting</subject><subject>Operons</subject><subject>Sporulation</subject><subject>Synthesis</subject><issn>0378-1097</issn><issn>1574-6968</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>1998</creationdate><recordtype>article</recordtype><recordid>eNqVkEFr2zAYhkVpoWnW_2DasZtdfZZk2TsMtpKsZSk5pD2LL7IcHBzLlWKW_PvJJGQwukJ10UHP-_LqIeQGaALh3K0TEJLHWZHlCRRFnmyXwIDzZHdGRqenczKiTOYx0EJekivv15RSntJsROaTXeeM97VtI1tFZYu_ImzLaOXsZDGLbGecbX1U9q5uV5HvrOsb3B7pH6jrpul9tDEr3BpX95tP5KLCxpvr4z0mL9PJ8_1DPJv_fLz_Pos1kwBxUfKqhJxrzfOlpqhLLoyUCCjoki05yCJH1CkYwKoyWg5QJShyMCxDYGPy5dDbOfvaG79Vm9pr0zTYGtt7BZngBQMRwNt_wLXtXRu2qZQFVZkAMdR9PVDaWe-dqVTn6g26vQKqBtNqrQadatCpBtPqaFrtQli8E6aQqcWgXw36VUhSmoamNOQ-H6eh19hUDltd-1M4ZVLkkAXs2wH7XTdm_4Fhavo0gxz-7rN99594_Na__gA7aLN3</recordid><startdate>199808</startdate><enddate>199808</enddate><creator>Hlaváček, Otakar</creator><creator>Adamec, Jiřı́</creator><creator>Vomastek, Tomáš</creator><creator>Babková, Lenka</creator><creator>Sedlák, Miroslav</creator><creator>Vohradský, Jiřı́</creator><creator>Váchová, Libuše</creator><creator>Chaloupka, Jiřı́</creator><general>Blackwell Publishing Ltd</general><general>Blackwell</general><general>Oxford University Press</general><scope>IQODW</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>3V.</scope><scope>7QL</scope><scope>7T7</scope><scope>7TK</scope><scope>7TM</scope><scope>7U9</scope><scope>7X7</scope><scope>7XB</scope><scope>88E</scope><scope>8AO</scope><scope>8C1</scope><scope>8FD</scope><scope>8FE</scope><scope>8FH</scope><scope>8FI</scope><scope>8FJ</scope><scope>8FK</scope><scope>ABUWG</scope><scope>AFKRA</scope><scope>AZQEC</scope><scope>BBNVY</scope><scope>BENPR</scope><scope>BHPHI</scope><scope>C1K</scope><scope>CCPQU</scope><scope>DWQXO</scope><scope>FR3</scope><scope>FYUFA</scope><scope>GHDGH</scope><scope>GNUQQ</scope><scope>H94</scope><scope>HCIFZ</scope><scope>K9.</scope><scope>LK8</scope><scope>M0S</scope><scope>M1P</scope><scope>M7N</scope><scope>M7P</scope><scope>P64</scope><scope>PQEST</scope><scope>PQQKQ</scope><scope>PQUKI</scope><scope>RC3</scope></search><sort><creationdate>199808</creationdate><title>Expression of dnaK and groESL operons during sporulation of Bacillus megaterium</title><author>Hlaváček, Otakar ; Adamec, Jiřı́ ; Vomastek, Tomáš ; Babková, Lenka ; Sedlák, Miroslav ; Vohradský, Jiřı́ ; Váchová, Libuše ; Chaloupka, Jiřı́</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c3711-9d4fd184cc48bc0acd45e77a1a50b3b41798aac21e1affec7bc0af50a41e36a13</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>1998</creationdate><topic>Bacillus megaterium</topic><topic>Bacteriology</topic><topic>Biological and medical sciences</topic><topic>Chaperones</topic><topic>DnaK protein</topic><topic>Fundamental and applied biological sciences. Psychology</topic><topic>Gene expression</topic><topic>Genetics</topic><topic>Heat shock</topic><topic>Heat shock protein</topic><topic>Microbiology</topic><topic>mRNA</topic><topic>Mutation</topic><topic>Northern blotting</topic><topic>Operons</topic><topic>Sporulation</topic><topic>Synthesis</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Hlaváček, Otakar</creatorcontrib><creatorcontrib>Adamec, Jiřı́</creatorcontrib><creatorcontrib>Vomastek, Tomáš</creatorcontrib><creatorcontrib>Babková, Lenka</creatorcontrib><creatorcontrib>Sedlák, Miroslav</creatorcontrib><creatorcontrib>Vohradský, Jiřı́</creatorcontrib><creatorcontrib>Váchová, Libuše</creatorcontrib><creatorcontrib>Chaloupka, Jiřı́</creatorcontrib><collection>Pascal-Francis</collection><collection>CrossRef</collection><collection>ProQuest Central (Corporate)</collection><collection>Bacteriology Abstracts (Microbiology B)</collection><collection>Industrial and Applied Microbiology Abstracts (Microbiology A)</collection><collection>Neurosciences Abstracts</collection><collection>Nucleic Acids Abstracts</collection><collection>Virology and AIDS Abstracts</collection><collection>ProQuest_Health & Medical Collection</collection><collection>ProQuest Central (purchase pre-March 2016)</collection><collection>Medical Database (Alumni Edition)</collection><collection>ProQuest Pharma Collection</collection><collection>Public Health Database</collection><collection>Technology Research Database</collection><collection>ProQuest SciTech Collection</collection><collection>ProQuest Natural Science Collection</collection><collection>Hospital Premium Collection</collection><collection>Hospital Premium Collection (Alumni Edition)</collection><collection>ProQuest Central (Alumni) (purchase pre-March 2016)</collection><collection>ProQuest Central (Alumni)</collection><collection>ProQuest Central UK/Ireland</collection><collection>ProQuest Central Essentials</collection><collection>Biological Science Collection</collection><collection>AUTh Library subscriptions: ProQuest Central</collection><collection>ProQuest Natural Science Collection</collection><collection>Environmental Sciences and Pollution Management</collection><collection>ProQuest One Community College</collection><collection>ProQuest Central</collection><collection>Engineering Research Database</collection><collection>Health Research Premium Collection</collection><collection>Health Research Premium Collection (Alumni)</collection><collection>ProQuest Central Student</collection><collection>AIDS and Cancer Research Abstracts</collection><collection>SciTech Premium Collection</collection><collection>ProQuest Health & Medical Complete (Alumni)</collection><collection>ProQuest Biological Science Collection</collection><collection>Health & Medical Collection (Alumni Edition)</collection><collection>Medical Database</collection><collection>Algology Mycology and Protozoology Abstracts (Microbiology C)</collection><collection>ProQuest Biological Science Journals</collection><collection>Biotechnology and BioEngineering Abstracts</collection><collection>ProQuest One Academic Eastern Edition (DO NOT USE)</collection><collection>ProQuest One Academic</collection><collection>ProQuest One Academic UKI Edition</collection><collection>Genetics Abstracts</collection><jtitle>FEMS microbiology letters</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Hlaváček, Otakar</au><au>Adamec, Jiřı́</au><au>Vomastek, Tomáš</au><au>Babková, Lenka</au><au>Sedlák, Miroslav</au><au>Vohradský, Jiřı́</au><au>Váchová, Libuše</au><au>Chaloupka, Jiřı́</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Expression of dnaK and groESL operons during sporulation of Bacillus megaterium</atitle><jtitle>FEMS microbiology letters</jtitle><date>1998-08</date><risdate>1998</risdate><volume>165</volume><issue>1</issue><spage>181</spage><epage>186</epage><pages>181-186</pages><issn>0378-1097</issn><eissn>1574-6968</eissn><coden>FMLED7</coden><abstract>Abstract
Expression of the dnaK and groEL genes during sporulation was assayed by determination of their mRNA levels by Northern blotting and compared with the relative level and rate of synthesis of the corresponding proteins. The ability of sporulating cells to respond to a heat shock by an increase in dnaK and groEL expression was determined at the same time. Synthesis of DnaK and GroEL encoding mRNAs during sporulation in non-shocked cells was low suggesting that this kind of cytodifferentiation was not accompanied by enhanced synthesis of these chaperones. Also the ability of sporulating cells to respond to a heat shock by stimulating their synthesis substantially decreased during the reversible and dropped to negligible values during the irreversible sporulation phase. Nevertheless, some dependence of the heat shock response on sporulation exists because sporulation suppression by mutation or by netropsin treatment further decreased the cells' capacity to respond to a heat shock.</abstract><cop>Oxford, UK</cop><pub>Blackwell Publishing Ltd</pub><doi>10.1111/j.1574-6968.1998.tb13144.x</doi><tpages>6</tpages></addata></record> |
| fulltext | fulltext |
| identifier | ISSN: 0378-1097 |
| ispartof | FEMS microbiology letters, 1998-08, Vol.165 (1), p.181-186 |
| issn | 0378-1097 1574-6968 |
| language | eng |
| recordid | cdi_proquest_miscellaneous_16549315 |
| source | Oxford Journals Online |
| subjects | Bacillus megaterium Bacteriology Biological and medical sciences Chaperones DnaK protein Fundamental and applied biological sciences. Psychology Gene expression Genetics Heat shock Heat shock protein Microbiology mRNA Mutation Northern blotting Operons Sporulation Synthesis |
| title | Expression of dnaK and groESL operons during sporulation of Bacillus megaterium |
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