Production of ice nuclei from two recombinant Zymomonas mobilis strains employing the inaZ gene of Pseudomonas syringae

A new recombinant plasmid, pBZIP1, was constructed for heterologous expression of high levels of ice nucleation activity in ethanol-producing Zymomonas mobilis strains CP4 and NCIB 11163. The plasmid construct contained the mobilization region and tetracycline resistance gene of pBR325, the replicat...

Full description

Saved in:
Bibliographic Details
Published in:Biotechnology letters 1998-07, Vol.20 (7), p.647-651
Main Authors: Varsaki, A, Afendra, A.S, Vartholomatos, G, Tegos, G, Drainas, C
Format: Article
Language:English
Subjects:
Bacteria
Biological and medical sciences
Biotechnology
Ethanol
Fundamental and applied biological sciences. Psychology
Genetic engineering
Genetic technics
Ice
ice nucleation
Methods. Procedures. Technologies
Microbiology
Modification of gene expression level
Nucleation
Pseudomonas syringae
Zymomonas mobilis
Citations: Items that cite this one
Online Access:Get full text
Tags: Add Tag
No Tags, Be the first to tag this record!
cited_by cdi_FETCH-LOGICAL-c310t-15966e80bcdc26737e8a9d1f74d1365cc77718f69c8da2c0386e97dddbbf1afd3
cites
container_end_page 651
container_issue 7
container_start_page 647
container_title Biotechnology letters
container_volume 20
creator Varsaki, A
Afendra, A.S
Vartholomatos, G
Tegos, G
Drainas, C
description A new recombinant plasmid, pBZIP1, was constructed for heterologous expression of high levels of ice nucleation activity in ethanol-producing Zymomonas mobilis strains CP4 and NCIB 11163. The plasmid construct contained the mobilization region and tetracycline resistance gene of pBR325, the replication region of the Z. mobilis native plasmid pZMO3 and the Pseudomonas syringae ice nucleation gene under the control of the Z. mobilis CP4 pyruvate decarboxylase promoter (PpdC). Z. mobilis transconjugants retained the plasmid stably, expressed ice nucleation activity up to 0.73 log [ice nuclei/cell] and can be used as improved sources of ice nuclei for industrial applications.
doi_str_mv 10.1023/A:1005310321474
format article
fullrecord <record><control><sourceid>proquest_pasca</sourceid><recordid>TN_cdi_proquest_miscellaneous_16555080</recordid><sourceformat>XML</sourceformat><sourcesystem>PC</sourcesystem><sourcerecordid>2101006781</sourcerecordid><originalsourceid>FETCH-LOGICAL-c310t-15966e80bcdc26737e8a9d1f74d1365cc77718f69c8da2c0386e97dddbbf1afd3</originalsourceid><addsrcrecordid>eNpdz01r3DAQgGFRWug2zTnHilJ6czojWZKdWwjNBwQaSHLJxcj62CrY0kayCfvvq5A99TSXZ15mCDlBOEVg_Nf5GQIIjsAZtqr9QDYoFG-kUvIj2QC22Ii2Z5_Jl1KeAaBXoDbk9S4nu5olpEiTp8E4GlczuUB9TjNdXhPNzqR5DFHHhT7t5zSnqAud0ximUGhZsg6xUDfvprQPcUuXv45W_US3Lrq36F1xqz2slX2uRruv5JPXU3HHh3lEHi9_P1xcN7d_rm4uzm8bUz9ZGhS9lK6D0VjDpOLKdbq36FVrkUthjFIKOy9701nNDPBOul5Za8fRo_aWH5Gf791dTi-rK8swh2LcNOno0loGlEII6KDC7__B57TmWG8bFBfYSgBV0Y8D0sXoyWcdTSjDLodZ5_3AWga1V9m3d-Z1GvQ2V_J4zwA5sK7Hliv-D2D2g2M</addsrcrecordid><sourcetype>Aggregation Database</sourcetype><iscdi>true</iscdi><recordtype>article</recordtype><pqid>735146007</pqid></control><display><type>article</type><title>Production of ice nuclei from two recombinant Zymomonas mobilis strains employing the inaZ gene of Pseudomonas syringae</title><source>Springer Nature</source><creator>Varsaki, A ; Afendra, A.S ; Vartholomatos, G ; Tegos, G ; Drainas, C</creator><creatorcontrib>Varsaki, A ; Afendra, A.S ; Vartholomatos, G ; Tegos, G ; Drainas, C</creatorcontrib><description>A new recombinant plasmid, pBZIP1, was constructed for heterologous expression of high levels of ice nucleation activity in ethanol-producing Zymomonas mobilis strains CP4 and NCIB 11163. The plasmid construct contained the mobilization region and tetracycline resistance gene of pBR325, the replication region of the Z. mobilis native plasmid pZMO3 and the Pseudomonas syringae ice nucleation gene under the control of the Z. mobilis CP4 pyruvate decarboxylase promoter (PpdC). Z. mobilis transconjugants retained the plasmid stably, expressed ice nucleation activity up to 0.73 log [ice nuclei/cell] and can be used as improved sources of ice nuclei for industrial applications.</description><identifier>ISSN: 0141-5492</identifier><identifier>EISSN: 1573-6776</identifier><identifier>DOI: 10.1023/A:1005310321474</identifier><identifier>CODEN: BILED3</identifier><language>eng</language><publisher>Dordrecht: Springer</publisher><subject>Bacteria ; Biological and medical sciences ; Biotechnology ; Ethanol ; Fundamental and applied biological sciences. Psychology ; Genetic engineering ; Genetic technics ; Ice ; ice nucleation ; Methods. Procedures. Technologies ; Microbiology ; Modification of gene expression level ; Nucleation ; Pseudomonas syringae ; Zymomonas mobilis</subject><ispartof>Biotechnology letters, 1998-07, Vol.20 (7), p.647-651</ispartof><rights>1998 INIST-CNRS</rights><rights>Chapman and Hall 1998</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c310t-15966e80bcdc26737e8a9d1f74d1365cc77718f69c8da2c0386e97dddbbf1afd3</citedby></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><link.rule.ids>314,778,782,27907,27908</link.rule.ids><backlink>$$Uhttp://pascal-francis.inist.fr/vibad/index.php?action=getRecordDetail&amp;idt=2420655$$DView record in Pascal Francis$$Hfree_for_read</backlink></links><search><creatorcontrib>Varsaki, A</creatorcontrib><creatorcontrib>Afendra, A.S</creatorcontrib><creatorcontrib>Vartholomatos, G</creatorcontrib><creatorcontrib>Tegos, G</creatorcontrib><creatorcontrib>Drainas, C</creatorcontrib><title>Production of ice nuclei from two recombinant Zymomonas mobilis strains employing the inaZ gene of Pseudomonas syringae</title><title>Biotechnology letters</title><description>A new recombinant plasmid, pBZIP1, was constructed for heterologous expression of high levels of ice nucleation activity in ethanol-producing Zymomonas mobilis strains CP4 and NCIB 11163. The plasmid construct contained the mobilization region and tetracycline resistance gene of pBR325, the replication region of the Z. mobilis native plasmid pZMO3 and the Pseudomonas syringae ice nucleation gene under the control of the Z. mobilis CP4 pyruvate decarboxylase promoter (PpdC). Z. mobilis transconjugants retained the plasmid stably, expressed ice nucleation activity up to 0.73 log [ice nuclei/cell] and can be used as improved sources of ice nuclei for industrial applications.</description><subject>Bacteria</subject><subject>Biological and medical sciences</subject><subject>Biotechnology</subject><subject>Ethanol</subject><subject>Fundamental and applied biological sciences. Psychology</subject><subject>Genetic engineering</subject><subject>Genetic technics</subject><subject>Ice</subject><subject>ice nucleation</subject><subject>Methods. Procedures. Technologies</subject><subject>Microbiology</subject><subject>Modification of gene expression level</subject><subject>Nucleation</subject><subject>Pseudomonas syringae</subject><subject>Zymomonas mobilis</subject><issn>0141-5492</issn><issn>1573-6776</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>1998</creationdate><recordtype>article</recordtype><recordid>eNpdz01r3DAQgGFRWug2zTnHilJ6czojWZKdWwjNBwQaSHLJxcj62CrY0kayCfvvq5A99TSXZ15mCDlBOEVg_Nf5GQIIjsAZtqr9QDYoFG-kUvIj2QC22Ii2Z5_Jl1KeAaBXoDbk9S4nu5olpEiTp8E4GlczuUB9TjNdXhPNzqR5DFHHhT7t5zSnqAud0ximUGhZsg6xUDfvprQPcUuXv45W_US3Lrq36F1xqz2slX2uRruv5JPXU3HHh3lEHi9_P1xcN7d_rm4uzm8bUz9ZGhS9lK6D0VjDpOLKdbq36FVrkUthjFIKOy9701nNDPBOul5Za8fRo_aWH5Gf791dTi-rK8swh2LcNOno0loGlEII6KDC7__B57TmWG8bFBfYSgBV0Y8D0sXoyWcdTSjDLodZ5_3AWga1V9m3d-Z1GvQ2V_J4zwA5sK7Hliv-D2D2g2M</recordid><startdate>19980701</startdate><enddate>19980701</enddate><creator>Varsaki, A</creator><creator>Afendra, A.S</creator><creator>Vartholomatos, G</creator><creator>Tegos, G</creator><creator>Drainas, C</creator><general>Springer</general><general>Springer Nature B.V</general><scope>FBQ</scope><scope>IQODW</scope><scope>3V.</scope><scope>7QL</scope><scope>7QR</scope><scope>7T7</scope><scope>7TB</scope><scope>7U5</scope><scope>7X7</scope><scope>7XB</scope><scope>88A</scope><scope>88E</scope><scope>88I</scope><scope>8AO</scope><scope>8FD</scope><scope>8FE</scope><scope>8FG</scope><scope>8FH</scope><scope>8FI</scope><scope>8FJ</scope><scope>8FK</scope><scope>ABJCF</scope><scope>ABUWG</scope><scope>AEUYN</scope><scope>AFKRA</scope><scope>AZQEC</scope><scope>BBNVY</scope><scope>BENPR</scope><scope>BGLVJ</scope><scope>BHPHI</scope><scope>C1K</scope><scope>CCPQU</scope><scope>DWQXO</scope><scope>FR3</scope><scope>FYUFA</scope><scope>GHDGH</scope><scope>GNUQQ</scope><scope>HCIFZ</scope><scope>K9.</scope><scope>L6V</scope><scope>L7M</scope><scope>LK8</scope><scope>M0S</scope><scope>M1P</scope><scope>M2P</scope><scope>M7N</scope><scope>M7P</scope><scope>M7S</scope><scope>P64</scope><scope>PQEST</scope><scope>PQQKQ</scope><scope>PQUKI</scope><scope>PTHSS</scope><scope>Q9U</scope><scope>7QO</scope></search><sort><creationdate>19980701</creationdate><title>Production of ice nuclei from two recombinant Zymomonas mobilis strains employing the inaZ gene of Pseudomonas syringae</title><author>Varsaki, A ; Afendra, A.S ; Vartholomatos, G ; Tegos, G ; Drainas, C</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c310t-15966e80bcdc26737e8a9d1f74d1365cc77718f69c8da2c0386e97dddbbf1afd3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>1998</creationdate><topic>Bacteria</topic><topic>Biological and medical sciences</topic><topic>Biotechnology</topic><topic>Ethanol</topic><topic>Fundamental and applied biological sciences. Psychology</topic><topic>Genetic engineering</topic><topic>Genetic technics</topic><topic>Ice</topic><topic>ice nucleation</topic><topic>Methods. Procedures. Technologies</topic><topic>Microbiology</topic><topic>Modification of gene expression level</topic><topic>Nucleation</topic><topic>Pseudomonas syringae</topic><topic>Zymomonas mobilis</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Varsaki, A</creatorcontrib><creatorcontrib>Afendra, A.S</creatorcontrib><creatorcontrib>Vartholomatos, G</creatorcontrib><creatorcontrib>Tegos, G</creatorcontrib><creatorcontrib>Drainas, C</creatorcontrib><collection>AGRIS</collection><collection>Pascal-Francis</collection><collection>ProQuest Central (Corporate)</collection><collection>Bacteriology Abstracts (Microbiology B)</collection><collection>Chemoreception Abstracts</collection><collection>Industrial and Applied Microbiology Abstracts (Microbiology A)</collection><collection>Mechanical &amp; Transportation Engineering Abstracts</collection><collection>Solid State and Superconductivity Abstracts</collection><collection>Health &amp; Medical Collection</collection><collection>ProQuest Central (purchase pre-March 2016)</collection><collection>Biology Database (Alumni Edition)</collection><collection>Medical Database (Alumni Edition)</collection><collection>Science Database (Alumni Edition)</collection><collection>ProQuest Pharma Collection</collection><collection>Technology Research Database</collection><collection>ProQuest SciTech Collection</collection><collection>ProQuest Technology Collection</collection><collection>ProQuest Natural Science Collection</collection><collection>Hospital Premium Collection</collection><collection>Hospital Premium Collection (Alumni Edition)</collection><collection>ProQuest Central (Alumni) (purchase pre-March 2016)</collection><collection>Materials Science &amp; Engineering Collection</collection><collection>ProQuest Central (Alumni)</collection><collection>ProQuest One Sustainability</collection><collection>ProQuest Central</collection><collection>ProQuest Central Essentials</collection><collection>Biological Science Collection</collection><collection>ProQuest Central</collection><collection>Technology Collection</collection><collection>Natural Science Collection</collection><collection>Environmental Sciences and Pollution Management</collection><collection>ProQuest One Community College</collection><collection>ProQuest Central Korea</collection><collection>Engineering Research Database</collection><collection>Health Research Premium Collection</collection><collection>Health Research Premium Collection (Alumni)</collection><collection>ProQuest Central Student</collection><collection>SciTech Premium Collection</collection><collection>ProQuest Health &amp; Medical Complete (Alumni)</collection><collection>ProQuest Engineering Collection</collection><collection>Advanced Technologies Database with Aerospace</collection><collection>ProQuest Biological Science Collection</collection><collection>Health &amp; Medical Collection (Alumni Edition)</collection><collection>Medical Database</collection><collection>ProQuest Science Journals</collection><collection>Algology Mycology and Protozoology Abstracts (Microbiology C)</collection><collection>Biological Science Database</collection><collection>Engineering Database</collection><collection>Biotechnology and BioEngineering Abstracts</collection><collection>ProQuest One Academic Eastern Edition (DO NOT USE)</collection><collection>ProQuest One Academic</collection><collection>ProQuest One Academic UKI Edition</collection><collection>Engineering Collection</collection><collection>ProQuest Central Basic</collection><collection>Biotechnology Research Abstracts</collection><jtitle>Biotechnology letters</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Varsaki, A</au><au>Afendra, A.S</au><au>Vartholomatos, G</au><au>Tegos, G</au><au>Drainas, C</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Production of ice nuclei from two recombinant Zymomonas mobilis strains employing the inaZ gene of Pseudomonas syringae</atitle><jtitle>Biotechnology letters</jtitle><date>1998-07-01</date><risdate>1998</risdate><volume>20</volume><issue>7</issue><spage>647</spage><epage>651</epage><pages>647-651</pages><issn>0141-5492</issn><eissn>1573-6776</eissn><coden>BILED3</coden><abstract>A new recombinant plasmid, pBZIP1, was constructed for heterologous expression of high levels of ice nucleation activity in ethanol-producing Zymomonas mobilis strains CP4 and NCIB 11163. The plasmid construct contained the mobilization region and tetracycline resistance gene of pBR325, the replication region of the Z. mobilis native plasmid pZMO3 and the Pseudomonas syringae ice nucleation gene under the control of the Z. mobilis CP4 pyruvate decarboxylase promoter (PpdC). Z. mobilis transconjugants retained the plasmid stably, expressed ice nucleation activity up to 0.73 log [ice nuclei/cell] and can be used as improved sources of ice nuclei for industrial applications.</abstract><cop>Dordrecht</cop><pub>Springer</pub><doi>10.1023/A:1005310321474</doi><tpages>5</tpages></addata></record>
fulltext fulltext
identifier ISSN: 0141-5492
ispartof Biotechnology letters, 1998-07, Vol.20 (7), p.647-651
issn 0141-5492
1573-6776
language eng
recordid cdi_proquest_miscellaneous_16555080
source Springer Nature
subjects Bacteria
Biological and medical sciences
Biotechnology
Ethanol
Fundamental and applied biological sciences. Psychology
Genetic engineering
Genetic technics
Ice
ice nucleation
Methods. Procedures. Technologies
Microbiology
Modification of gene expression level
Nucleation
Pseudomonas syringae
Zymomonas mobilis
title Production of ice nuclei from two recombinant Zymomonas mobilis strains employing the inaZ gene of Pseudomonas syringae
url http://sfxeu10.hosted.exlibrisgroup.com/loughborough?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&ctx_tim=2025-01-16T17%3A07%3A14IST&url_ver=Z39.88-2004&url_ctx_fmt=infofi/fmt:kev:mtx:ctx&rfr_id=info:sid/primo.exlibrisgroup.com:primo3-Article-proquest_pasca&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.atitle=Production%20of%20ice%20nuclei%20from%20two%20recombinant%20Zymomonas%20mobilis%20strains%20employing%20the%20inaZ%20gene%20of%20Pseudomonas%20syringae&rft.jtitle=Biotechnology%20letters&rft.au=Varsaki,%20A&rft.date=1998-07-01&rft.volume=20&rft.issue=7&rft.spage=647&rft.epage=651&rft.pages=647-651&rft.issn=0141-5492&rft.eissn=1573-6776&rft.coden=BILED3&rft_id=info:doi/10.1023/A:1005310321474&rft_dat=%3Cproquest_pasca%3E2101006781%3C/proquest_pasca%3E%3Cgrp_id%3Ecdi_FETCH-LOGICAL-c310t-15966e80bcdc26737e8a9d1f74d1365cc77718f69c8da2c0386e97dddbbf1afd3%3C/grp_id%3E%3Coa%3E%3C/oa%3E%3Curl%3E%3C/url%3E&rft_id=info:oai/&rft_pqid=735146007&rft_id=info:pmid/&rfr_iscdi=true