Up Regulation of Liver-enriched Transcription Factors HNF4a and HNF6 and Liver-Specific MicroRNA (miR-122) by Inhibition of Let-7b in Mesenchymal Stem Cells

MicroRNAs are small non‐coding RNAs that regulate key processes of the stem cells. Although, microRNAs have emerged as powerful regulators of differentiation, few studies have been focused on the post‐transcriptional regulation of hepatic differentiation in mesenchymal stem cells (MSCs) by microRNAs...

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Bibliographic Details
Published in:Chemical biology & drug design 2015-03, Vol.85 (3), p.268-279
Main Authors: Alizadeh, Effat, Akbarzadeh, Abolfazl, Eslaminejad, Mohamadreza Baghaban, Barzegar, Abolfazl, Hashemzadeh, Shahryar, Nejati-Koshki, Kazem, Zarghami, Nosratollah
Format: Article
Language:English
Subjects:
adipose tissue
Adipose Tissue - cytology
Adult
Cell Differentiation
Cell Survival
Cells, Cultured
Female
G1 Phase Cell Cycle Checkpoints
Hep G2 Cells
Hepatocyte Nuclear Factor 4 - genetics
Hepatocyte Nuclear Factor 4 - metabolism
Hepatocyte Nuclear Factor 6 - genetics
Hepatocyte Nuclear Factor 6 - metabolism
Hepatocytes - cytology
Hepatocytes - metabolism
hepatogenic differentiation
HNF4α
HNF6
Humans
inhibition
Let-7b
Liver - metabolism
Male
mesenchymal stem cells
Mesenchymal Stromal Cells - cytology
Mesenchymal Stromal Cells - metabolism
MicroRNAs - genetics
MicroRNAs - metabolism
Middle Aged
miR-122
Oligonucleotides, Antisense - genetics
Oligonucleotides, Antisense - metabolism
Up-Regulation
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Summary:MicroRNAs are small non‐coding RNAs that regulate key processes of the stem cells. Although, microRNAs have emerged as powerful regulators of differentiation, few studies have been focused on the post‐transcriptional regulation of hepatic differentiation in mesenchymal stem cells (MSCs) by microRNAs. The aim of this study was to evaluate the specific effect of let‐7 microRNAs in particular let‐7b in hepatic commitment of human adipose tissue‐derived mesenchymal stem cells (hAT‐MSCs). The dynamic expression profile of let‐7a, b, c microRNAs and two liver‐enriched transcription factors (LETFs) HNF4a and HNF6 was studied during in vitro hepatic differentiation of hAT‐MSCs. Let‐7b was used for transient overexpression and knockdown investigations. It was shown that the expression of LETFs is inversely correlated with those of let‐7 miRNAs during differentiation progress (p 
ISSN:1747-0277
1747-0285
DOI:10.1111/cbdd.12398