The Short-Term Effects of C-Peptide on the Early Diabetes-Related Ultrastructural Changes to the Podocyte Slit Diaphragm of Glomeruli in Rats

Objective This study aimed to investigate the structural changes in the slit diaphragm, caused by early diabetes, and the nephroprotective effect of C‐peptide. Methods Streptozotocin‐induced type 1 diabetic Wistar rats were divided into control, control plus C‐peptide, early diabetes, and early diab...

Full description

Saved in:
Bibliographic Details
Published in:Microcirculation (New York, N.Y. 1994) N.Y. 1994), 2015-02, Vol.22 (2), p.122-132
Main Authors: Nakamoto, Hiroshi, Nakayama, Kazuhiko, Emoto, Noriaki, Kajiya, Fumihiko
Format: Article
Language:English
Subjects:
Adaptor Proteins, Signal Transducing - metabolism
Animals
C-peptide
C-Peptide - pharmacology
Cytoskeletal Proteins - metabolism
diabetes
Diabetes Mellitus, Experimental - drug therapy
Diabetes Mellitus, Experimental - metabolism
Diabetes Mellitus, Experimental - pathology
Diabetic Nephropathies - drug therapy
Diabetic Nephropathies - metabolism
Diabetic Nephropathies - pathology
diaphragm
electron microscopy
Glomerular Basement Membrane - metabolism
Glomerular Basement Membrane - ultrastructure
immunofluorescence
Intracellular Signaling Peptides and Proteins - metabolism
Male
Membrane Proteins - metabolism
Podocytes - pathology
Podocytes - ultrastructure
Rats
Rats, Wistar
slit podocyte
Citations: Items that this one cites
Items that cite this one
Online Access:Get full text
Tags: Add Tag
No Tags, Be the first to tag this record!
Description
Summary:Objective This study aimed to investigate the structural changes in the slit diaphragm, caused by early diabetes, and the nephroprotective effect of C‐peptide. Methods Streptozotocin‐induced type 1 diabetic Wistar rats were divided into control, control plus C‐peptide, early diabetes, and early diabetes plus C‐peptide groups. C‐peptide was infused into rats for one day. The slit diaphragm component proteins podocin, CD2AP, and nephrin, were stained immunofluorescently and their distribution quantitatively analyzed by determining the overlapping area ratio. The interfoot process gap length was measured from electron microscopic images. Results Diabetic duration correlated best with the area ratio of podocin and CD2AP (r = 0.626), followed by other protein combinations, showing progressive change in the slit diaphragm structure. C‐peptide‐treatment did not alter area ratios. The interfoot process gap length was wider in diabetic rats (p 
ISSN:1073-9688
1549-8719
DOI:10.1111/micc.12185