Clinicopathologic correlations of the BRAFV600E mutation, BRAF V600E immunohistochemistry, and BRAF RNA in situ hybridization in papillary thyroid carcinoma

The BRAFV600E mutation is the most common genetic alteration in papillary thyroid carcinoma (PTC). The aim of this study is to analyze the clinicopathologic correlations of the BRAFV600E mutation, BRAF V600E immunohistochemistry (IHC) and BRAF RNA in situ hybridization (ISH) in PTC. This study inclu...

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Published in:Pathology, research and practice research and practice, 2015-02, Vol.211 (2), p.162-170
Main Authors: Jung, Yoon Yang, Yoo, Jae Hyung, Park, Eon Sub, Kim, Mi Kyung, Lee, Tae Jin, Cho, Bo Youn, Chung, Yun Jae, Kang, Kyung Ho, Ahn, Hwa Young, Kim, Hee Sung
Format: Article
Language:English
Subjects:
Adolescent
Adult
Aged
Biomarkers, Tumor - genetics
BRAF V600E mutation
Carcinoma - genetics
Carcinoma - pathology
Carcinoma, Papillary
Female
Humans
Immunohistochemistry
In Situ Hybridization
Lymphatic Metastasis
Male
Middle Aged
Mutation
Papillary carcinoma
Prognosis
Proto-Oncogene Proteins B-raf - genetics
Real-Time Polymerase Chain Reaction
RNA - analysis
RNA in situ hybridization
Sensitivity and Specificity
Thyroid Cancer, Papillary
Thyroid Neoplasms - genetics
Thyroid Neoplasms - pathology
Tissue Array Analysis
Young Adult
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cited_by cdi_FETCH-LOGICAL-c268t-9919419769d5ac3a6029257ce8e48bd398770a2423e3b616bc511f00a11f4b953
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creator Jung, Yoon Yang
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Ahn, Hwa Young
Kim, Hee Sung
description The BRAFV600E mutation is the most common genetic alteration in papillary thyroid carcinoma (PTC). The aim of this study is to analyze the clinicopathologic correlations of the BRAFV600E mutation, BRAF V600E immunohistochemistry (IHC) and BRAF RNA in situ hybridization (ISH) in PTC. This study included 467 patients with PTC who underwent surgical resection. We studied the BRAFV600E mutation using real-time PCR and BRAF V600E and BRAF RNA ISH using tissue microarray (TMA). The frequencies of a positive BRAFV600E mutation by real-time PCR, positive BRAF V600E IHC, and high BRAF RNA ISH were 84%, 86%, and 70%, respectively, in PTC. Conventional PTC had higher positive rates in all three tests than other histologic types. The BRAFV600E mutation, BRAF V600E IHC, low ΔCt, and high BRAF RNA ISH were significantly associated with lymph node metastasis. The BRAFV600E mutation was significantly associated with positive immunostaining for BRAF V600E mutant protein (P
doi_str_mv 10.1016/j.prp.2014.10.005
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The aim of this study is to analyze the clinicopathologic correlations of the BRAFV600E mutation, BRAF V600E immunohistochemistry (IHC) and BRAF RNA in situ hybridization (ISH) in PTC. This study included 467 patients with PTC who underwent surgical resection. We studied the BRAFV600E mutation using real-time PCR and BRAF V600E and BRAF RNA ISH using tissue microarray (TMA). The frequencies of a positive BRAFV600E mutation by real-time PCR, positive BRAF V600E IHC, and high BRAF RNA ISH were 84%, 86%, and 70%, respectively, in PTC. Conventional PTC had higher positive rates in all three tests than other histologic types. The BRAFV600E mutation, BRAF V600E IHC, low ΔCt, and high BRAF RNA ISH were significantly associated with lymph node metastasis. The BRAFV600E mutation was significantly associated with positive immunostaining for BRAF V600E mutant protein (P&lt;0.001) overall, with high BRAF RNA ISH only in the follicular variant (P=0.035). No significant correlation was noted between BRAF V600E IHC and BRAF RNA ISH. The sensitivity of BRAF V600E IHC for the BRAFV600E mutation was 95%, and the specificity was 61% overall, 96% and 54% in the conventional type, and 85% and 70% in the follicular variant. Our results showed that positive BRAF V600E IHC significantly correlated with the BRAFV600E mutation. This suggests its clinical utility as a screening tool for the BRAFV600E mutation. 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The aim of this study is to analyze the clinicopathologic correlations of the BRAFV600E mutation, BRAF V600E immunohistochemistry (IHC) and BRAF RNA in situ hybridization (ISH) in PTC. This study included 467 patients with PTC who underwent surgical resection. We studied the BRAFV600E mutation using real-time PCR and BRAF V600E and BRAF RNA ISH using tissue microarray (TMA). The frequencies of a positive BRAFV600E mutation by real-time PCR, positive BRAF V600E IHC, and high BRAF RNA ISH were 84%, 86%, and 70%, respectively, in PTC. Conventional PTC had higher positive rates in all three tests than other histologic types. The BRAFV600E mutation, BRAF V600E IHC, low ΔCt, and high BRAF RNA ISH were significantly associated with lymph node metastasis. The BRAFV600E mutation was significantly associated with positive immunostaining for BRAF V600E mutant protein (P&lt;0.001) overall, with high BRAF RNA ISH only in the follicular variant (P=0.035). No significant correlation was noted between BRAF V600E IHC and BRAF RNA ISH. The sensitivity of BRAF V600E IHC for the BRAFV600E mutation was 95%, and the specificity was 61% overall, 96% and 54% in the conventional type, and 85% and 70% in the follicular variant. Our results showed that positive BRAF V600E IHC significantly correlated with the BRAFV600E mutation. This suggests its clinical utility as a screening tool for the BRAFV600E mutation. In addition, a high BRAF RNA ISH score could be a candidate marker of aggressive behavior in BRAFV600E mutation-positive cases of PTC.</abstract><cop>Germany</cop><pub>Elsevier GmbH</pub><pmid>25468810</pmid><doi>10.1016/j.prp.2014.10.005</doi><tpages>9</tpages></addata></record>
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ispartof Pathology, research and practice, 2015-02, Vol.211 (2), p.162-170
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1618-0631
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source Elsevier
subjects Adolescent
Adult
Aged
Biomarkers, Tumor - genetics
BRAF V600E mutation
Carcinoma - genetics
Carcinoma - pathology
Carcinoma, Papillary
Female
Humans
Immunohistochemistry
In Situ Hybridization
Lymphatic Metastasis
Male
Middle Aged
Mutation
Papillary carcinoma
Prognosis
Proto-Oncogene Proteins B-raf - genetics
Real-Time Polymerase Chain Reaction
RNA - analysis
RNA in situ hybridization
Sensitivity and Specificity
Thyroid Cancer, Papillary
Thyroid Neoplasms - genetics
Thyroid Neoplasms - pathology
Tissue Array Analysis
Young Adult
title Clinicopathologic correlations of the BRAFV600E mutation, BRAF V600E immunohistochemistry, and BRAF RNA in situ hybridization in papillary thyroid carcinoma
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