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Intrinsically disordered proteins as crucial constituents of cellular aqueous two phase systems and coacervates
•Eukaryotic cells contain various membrane-less organelles.•Such organelles can be generated due to the liquid–liquid phase separations.•Membrane-less organelles represent cellular aqueous two phase systems (ATPSes).•Intrinsically disordered proteins (IDPs) are crucial for generation of these organe...
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Published in: | FEBS letters 2015-01, Vol.589 (1), p.15-22 |
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creator | Uversky, Vladimir N. Kuznetsova, Irina M. Turoverov, Konstantin K. Zaslavsky, Boris |
description | •Eukaryotic cells contain various membrane-less organelles.•Such organelles can be generated due to the liquid–liquid phase separations.•Membrane-less organelles represent cellular aqueous two phase systems (ATPSes).•Intrinsically disordered proteins (IDPs) are crucial for generation of these organelles.
Here, we hypothesize that intrinsically disordered proteins (IDPs) serve as important drivers of the intracellular liquid–liquid phase separations that generate various membrane-less organelles. This hypothesis is supported by the overwhelming abundance of IDPs in these organelles. Assembly and disassembly of these organelles are controlled by changes in the concentrations of IDPs, their posttranslational modifications, binding of specific partners, and changes in the pH and/or temperature of the solution. Each resulting phase provides a distinct solvent environment for other solutes leading to their unequal distribution within phases. The specificity and efficiency of such partitioning is determined by the nature of the IDP(s) and defines “targeted” enrichment of specific molecules in the resulting membrane-less organelles that determines their specific activities. |
doi_str_mv | 10.1016/j.febslet.2014.11.028 |
format | article |
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Here, we hypothesize that intrinsically disordered proteins (IDPs) serve as important drivers of the intracellular liquid–liquid phase separations that generate various membrane-less organelles. This hypothesis is supported by the overwhelming abundance of IDPs in these organelles. Assembly and disassembly of these organelles are controlled by changes in the concentrations of IDPs, their posttranslational modifications, binding of specific partners, and changes in the pH and/or temperature of the solution. Each resulting phase provides a distinct solvent environment for other solutes leading to their unequal distribution within phases. The specificity and efficiency of such partitioning is determined by the nature of the IDP(s) and defines “targeted” enrichment of specific molecules in the resulting membrane-less organelles that determines their specific activities.</description><identifier>ISSN: 0014-5793</identifier><identifier>EISSN: 1873-3468</identifier><identifier>DOI: 10.1016/j.febslet.2014.11.028</identifier><identifier>PMID: 25436423</identifier><language>eng</language><publisher>England: Elsevier B.V</publisher><subject>Aqueous two-phase system ; Coacervate ; Intrinsically disordered proteins ; Liquid–liquid phase transition ; Membrane-less organelles ; Organelle Biogenesis ; Organelles - physiology ; Partitioning ; Protein Processing, Post-Translational - physiology ; Proteins - physiology</subject><ispartof>FEBS letters, 2015-01, Vol.589 (1), p.15-22</ispartof><rights>2014 Federation of European Biochemical Societies</rights><rights>FEBS Letters 589 (2015) 1873-3468 © 2015 Federation of European Biochemical Societies</rights><rights>Copyright © 2014 Federation of European Biochemical Societies. Published by Elsevier B.V. All rights reserved.</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c5103-d4bf1f9e477bb5223fabc2a187387e170bfbda0dba70ef19b9a0665e1da12d873</citedby><cites>FETCH-LOGICAL-c5103-d4bf1f9e477bb5223fabc2a187387e170bfbda0dba70ef19b9a0665e1da12d873</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktohtml>$$Uhttps://www.sciencedirect.com/science/article/pii/S0014579314008333$$EHTML$$P50$$Gelsevier$$Hfree_for_read</linktohtml><link.rule.ids>314,780,784,3549,27924,27925,45780</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/25436423$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Uversky, Vladimir N.</creatorcontrib><creatorcontrib>Kuznetsova, Irina M.</creatorcontrib><creatorcontrib>Turoverov, Konstantin K.</creatorcontrib><creatorcontrib>Zaslavsky, Boris</creatorcontrib><title>Intrinsically disordered proteins as crucial constituents of cellular aqueous two phase systems and coacervates</title><title>FEBS letters</title><addtitle>FEBS Lett</addtitle><description>•Eukaryotic cells contain various membrane-less organelles.•Such organelles can be generated due to the liquid–liquid phase separations.•Membrane-less organelles represent cellular aqueous two phase systems (ATPSes).•Intrinsically disordered proteins (IDPs) are crucial for generation of these organelles.
Here, we hypothesize that intrinsically disordered proteins (IDPs) serve as important drivers of the intracellular liquid–liquid phase separations that generate various membrane-less organelles. This hypothesis is supported by the overwhelming abundance of IDPs in these organelles. Assembly and disassembly of these organelles are controlled by changes in the concentrations of IDPs, their posttranslational modifications, binding of specific partners, and changes in the pH and/or temperature of the solution. Each resulting phase provides a distinct solvent environment for other solutes leading to their unequal distribution within phases. The specificity and efficiency of such partitioning is determined by the nature of the IDP(s) and defines “targeted” enrichment of specific molecules in the resulting membrane-less organelles that determines their specific activities.</description><subject>Aqueous two-phase system</subject><subject>Coacervate</subject><subject>Intrinsically disordered proteins</subject><subject>Liquid–liquid phase transition</subject><subject>Membrane-less organelles</subject><subject>Organelle Biogenesis</subject><subject>Organelles - physiology</subject><subject>Partitioning</subject><subject>Protein Processing, Post-Translational - physiology</subject><subject>Proteins - physiology</subject><issn>0014-5793</issn><issn>1873-3468</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2015</creationdate><recordtype>article</recordtype><recordid>eNqNkU9v1DAQxS0EokvhI4B85JLFYydxckJQtbRSJQ7A2fKfifAqmywep9V--zrahSs9Wda89_zGP8beg9iCgPbTbjugoxHzVgqotwBbIbsXbAOdVpWq2-4l24gyqRrdqwv2hmgnyr2D_jW7kE2t2lqqDZvvppziRNHbcTzyEGlOARMGfkhzxjLhlrhPi4925H6eKMe84JSJzwP3OI7LaBO3fxacF-L5ceaH35aQ05Ey7ot7CsVmPaYHm5HesleDHQnfnc9L9uvm-ufVbXX__dvd1Zf7yjcgVBVqN8DQY621c42UarDOS7su12kELdzgghXBWS1wgN71VrRtgxAsyFBUl-zjKbesUbpRNvtIa107rUUNtE2ja9DNc6Sq77XuQRZpc5L6NBMlHMwhxb1NRwPCrFjMzpyxmBWLATAFS_F9OD-xuD2Gf66_HIrg9iR4jCMen5dqbq6_yh8r4xUx1EJ0Sq1Rn09RWL73IWIy5CNOHkNM6LMJc_xP2yfw5blK</recordid><startdate>20150102</startdate><enddate>20150102</enddate><creator>Uversky, Vladimir N.</creator><creator>Kuznetsova, Irina M.</creator><creator>Turoverov, Konstantin K.</creator><creator>Zaslavsky, Boris</creator><general>Elsevier B.V</general><scope>6I.</scope><scope>AAFTH</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope><scope>7ST</scope><scope>C1K</scope><scope>SOI</scope></search><sort><creationdate>20150102</creationdate><title>Intrinsically disordered proteins as crucial constituents of cellular aqueous two phase systems and coacervates</title><author>Uversky, Vladimir N. ; Kuznetsova, Irina M. ; Turoverov, Konstantin K. ; Zaslavsky, Boris</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c5103-d4bf1f9e477bb5223fabc2a187387e170bfbda0dba70ef19b9a0665e1da12d873</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2015</creationdate><topic>Aqueous two-phase system</topic><topic>Coacervate</topic><topic>Intrinsically disordered proteins</topic><topic>Liquid–liquid phase transition</topic><topic>Membrane-less organelles</topic><topic>Organelle Biogenesis</topic><topic>Organelles - physiology</topic><topic>Partitioning</topic><topic>Protein Processing, Post-Translational - physiology</topic><topic>Proteins - physiology</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Uversky, Vladimir N.</creatorcontrib><creatorcontrib>Kuznetsova, Irina M.</creatorcontrib><creatorcontrib>Turoverov, Konstantin K.</creatorcontrib><creatorcontrib>Zaslavsky, Boris</creatorcontrib><collection>ScienceDirect Open Access Titles</collection><collection>Elsevier:ScienceDirect:Open Access</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>MEDLINE - Academic</collection><collection>Environment Abstracts</collection><collection>Environmental Sciences and Pollution Management</collection><collection>Environment Abstracts</collection><jtitle>FEBS letters</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Uversky, Vladimir N.</au><au>Kuznetsova, Irina M.</au><au>Turoverov, Konstantin K.</au><au>Zaslavsky, Boris</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Intrinsically disordered proteins as crucial constituents of cellular aqueous two phase systems and coacervates</atitle><jtitle>FEBS letters</jtitle><addtitle>FEBS Lett</addtitle><date>2015-01-02</date><risdate>2015</risdate><volume>589</volume><issue>1</issue><spage>15</spage><epage>22</epage><pages>15-22</pages><issn>0014-5793</issn><eissn>1873-3468</eissn><abstract>•Eukaryotic cells contain various membrane-less organelles.•Such organelles can be generated due to the liquid–liquid phase separations.•Membrane-less organelles represent cellular aqueous two phase systems (ATPSes).•Intrinsically disordered proteins (IDPs) are crucial for generation of these organelles.
Here, we hypothesize that intrinsically disordered proteins (IDPs) serve as important drivers of the intracellular liquid–liquid phase separations that generate various membrane-less organelles. This hypothesis is supported by the overwhelming abundance of IDPs in these organelles. Assembly and disassembly of these organelles are controlled by changes in the concentrations of IDPs, their posttranslational modifications, binding of specific partners, and changes in the pH and/or temperature of the solution. Each resulting phase provides a distinct solvent environment for other solutes leading to their unequal distribution within phases. The specificity and efficiency of such partitioning is determined by the nature of the IDP(s) and defines “targeted” enrichment of specific molecules in the resulting membrane-less organelles that determines their specific activities.</abstract><cop>England</cop><pub>Elsevier B.V</pub><pmid>25436423</pmid><doi>10.1016/j.febslet.2014.11.028</doi><tpages>8</tpages><oa>free_for_read</oa></addata></record> |
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subjects | Aqueous two-phase system Coacervate Intrinsically disordered proteins Liquid–liquid phase transition Membrane-less organelles Organelle Biogenesis Organelles - physiology Partitioning Protein Processing, Post-Translational - physiology Proteins - physiology |
title | Intrinsically disordered proteins as crucial constituents of cellular aqueous two phase systems and coacervates |
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