Biodegradation of atrazine under denitrifying conditions

Anaerobic biodegradation of atrazine by the bacterial isolate M91-3 was characterized with respect to mineralization, metabolite formation, and denitrification. The ability of the isolate to enhance atrazine biodegradation in anaerobic sediment slurries was also investigated. The organism utilized a...

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Bibliographic Details
Published in:Applied microbiology and biotechnology 1998-05, Vol.49 (5), p.618-623
Main Authors: Crawford, J.J, Sims, G.K, Mulvaney, R.L, Radosevich, M
Format: Article
Language:English
Subjects:
Anaerobic biodegradation
Anoxic conditions
Atrazine
Atrazine - metabolism
Bacteria
Biodegradation
Biodegradation of pollutants
Biodegradation, Environmental
Biological and medical sciences
Bioreactors
bioremediation
Biotechnology
Biotransformation
Carbon Dioxide - metabolism
Chromatography, High Pressure Liquid
Denitrification
Environment and pollution
Fundamental and applied biological sciences. Psychology
Herbicides
Herbicides - metabolism
Industrial applications and implications. Economical aspects
Liquid chromatography
Metabolites
Microbiology
Mineralization
Sediments
Slurries
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Summary:Anaerobic biodegradation of atrazine by the bacterial isolate M91-3 was characterized with respect to mineralization, metabolite formation, and denitrification. The ability of the isolate to enhance atrazine biodegradation in anaerobic sediment slurries was also investigated. The organism utilized atrazine as its sole source of carbon and nitrogen under anoxic conditions in fixed-film (glass beads) batch column systems. Results of HPLC and TLC radiochromatography suggested that anaerobic biotransformation of atrazine by microbial isolate M91-3 involved hydroxyatrazine formation. Ring cleavage was demonstrated by 14CO2 evolution. Denitrification was confirmed by detection of 15N2 in head-space samples of K15NO3-amended anaerobic liquid cultures. In aquatic sediments, mineralization of uniformly ring-labeled [14C]atrazine occurred in both M91-3-inoculated and uninoculated sediment. Inoculation of sediments with M91-3 did not significantly enhance anaerobic mineralization of atrazine as compared to uninoculated sediment, which suggests the presence of indigenous organisms capable of anaerobic atrazine biodegradation. Results of this study suggest that the use of M91-3 in a fixed-film bioreactor may have applications in the anaerobic removal of atrazine and nitrate from aqueous media.
ISSN:0175-7598
1432-0614
DOI:10.1007/s002530051223