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alpha-D-galactosidase (EC 3.2.1.22) from Bifidobacterium longum
α‐Galactosidase activity in Bifidobacterium longum was maximal in a range of 40–45°C and at pH of 5.8. At temperatures above 60°C the enzyme was completely inactivated, but it maintained a 100% activity during storage at low temperatures (4, ‐ 30, ‐ 70°C) for 3 months. The addition of Hg2+ to the re...
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Published in: | Letters in applied microbiology 1994-07, Vol.19 (1), p.16-19 |
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Main Authors: | , , , |
Format: | Article |
Language: | English |
Subjects: | |
Citations: | Items that this one cites Items that cite this one |
Online Access: | Get full text |
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Summary: | α‐Galactosidase activity in Bifidobacterium longum was maximal in a range of 40–45°C and at pH of 5.8. At temperatures above 60°C the enzyme was completely inactivated, but it maintained a 100% activity during storage at low temperatures (4, ‐ 30, ‐ 70°C) for 3 months. The addition of Hg2+ to the reaction buffer produced a strong inhibitory effect while Mn2+ exerted a slight positive effect upon activity. The addition of EDTA, inhibitors and other metal ions had no effect on the enzyme α‐galactosidase. |
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ISSN: | 0266-8254 1472-765X |
DOI: | 10.1111/j.1472-765X.1994.tb00892.x |