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IL-4 and IL-13 have overlapping but distinct effects on HIV production in monocytes
In HIV‐1‐infected monocytes and monocytoid cell lines, viral expression can be observed as high‐level production, restricted (chronic low‐level) expression, and latency (no viral expression). Interleukin‐13 (IL‐13) and IL‐4, which have remarkedly similar deactivating effects on inflammatory monocyte...
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Published in: | Journal of leukocyte biology 1994-09, Vol.56 (3), p.340-346 |
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container_end_page | 346 |
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container_title | Journal of leukocyte biology |
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creator | Mikovits, Judy A. Meyers, Anne M. Ortaldo, John R. Minty, Adrian Caput, Daniel Ferrara, Pascual Ruscetti, Francis W. |
description | In HIV‐1‐infected monocytes and monocytoid cell lines, viral expression can be observed as high‐level production, restricted (chronic low‐level) expression, and latency (no viral expression). Interleukin‐13 (IL‐13) and IL‐4, which have remarkedly similar deactivating effects on inflammatory monocyte functions, were studied for their regulation of HIV expression in monocytes. Pretreatment of peripheral monocytes for 48–72 h with IL‐13 markedly decreased acute HIV infection, whereas IL‐4 increased it. Similar effects were seen when the U1 and R‐THP‐1 monocytoid cell lines with restricted HIV expression were treated with these cytokines. However, when these continuously producing cell lines were chronically treated with cytokines, IL‐13 increased HIV production. Neither IL‐4 nor IL‐13 stimulated HIV expression in latently infected cells. In chronically infected cells, several cytokines reduced viral mRNA. Both IL‐4 and IL‐13 increased monocyte aggregate formation, but only IL‐4 ultimately stimulated cytolysis of HIV‐infected monocytes as well as increased apoptosis of U1. In the presence of tumor necrosis factor α or IL‐6, which upregulate HIV expression, IL‐13 could no longer suppress HIV expression. These results indicate that IL‐4 and IL‐13, although closely related in modulating monocyte function, can have divergent effects on HIV expression in monocytes. Collectively, these data suggest that there exists a complex cytokine tissue environment with positive regulators of HIV expression able to override negative regulators. J. Leukoc. Biol. 56: 340–346; 1994. |
doi_str_mv | 10.1002/jlb.56.3.340 |
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Interleukin‐13 (IL‐13) and IL‐4, which have remarkedly similar deactivating effects on inflammatory monocyte functions, were studied for their regulation of HIV expression in monocytes. Pretreatment of peripheral monocytes for 48–72 h with IL‐13 markedly decreased acute HIV infection, whereas IL‐4 increased it. Similar effects were seen when the U1 and R‐THP‐1 monocytoid cell lines with restricted HIV expression were treated with these cytokines. However, when these continuously producing cell lines were chronically treated with cytokines, IL‐13 increased HIV production. Neither IL‐4 nor IL‐13 stimulated HIV expression in latently infected cells. In chronically infected cells, several cytokines reduced viral mRNA. Both IL‐4 and IL‐13 increased monocyte aggregate formation, but only IL‐4 ultimately stimulated cytolysis of HIV‐infected monocytes as well as increased apoptosis of U1. In the presence of tumor necrosis factor α or IL‐6, which upregulate HIV expression, IL‐13 could no longer suppress HIV expression. These results indicate that IL‐4 and IL‐13, although closely related in modulating monocyte function, can have divergent effects on HIV expression in monocytes. Collectively, these data suggest that there exists a complex cytokine tissue environment with positive regulators of HIV expression able to override negative regulators. J. Leukoc. Biol. 56: 340–346; 1994.</description><identifier>ISSN: 0741-5400</identifier><identifier>EISSN: 1938-3673</identifier><identifier>DOI: 10.1002/jlb.56.3.340</identifier><identifier>PMID: 7916030</identifier><language>eng</language><publisher>United States: Society for Leukocyte Biology</publisher><subject>Cells, Cultured ; cytokines ; DNA, Viral - analysis ; DNA, Viral - genetics ; Gene Expression Regulation, Viral - drug effects ; HIV - genetics ; HIV - isolation & purification ; HIV regulation ; human immunodeficiency virus 1 ; Humans ; Interleukin-13 ; Interleukin-4 - pharmacology ; Interleukin-6 - pharmacology ; Interleukins - pharmacology ; monocytes ; Monocytes - chemistry ; Monocytes - cytology ; Monocytes - microbiology ; RNA, Messenger - analysis ; RNA, Messenger - genetics ; RNA, Viral - analysis ; RNA, Viral - genetics ; Tumor Necrosis Factor-alpha - pharmacology</subject><ispartof>Journal of leukocyte biology, 1994-09, Vol.56 (3), p.340-346</ispartof><rights>1994 Society for Leukocyte Biology</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c3210-9060455615d6b941b758fa452c38d59907f309aac8199691d89e398cf8e3b7f53</citedby></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><link.rule.ids>314,776,780,27900,27901</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/7916030$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Mikovits, Judy A.</creatorcontrib><creatorcontrib>Meyers, Anne M.</creatorcontrib><creatorcontrib>Ortaldo, John R.</creatorcontrib><creatorcontrib>Minty, Adrian</creatorcontrib><creatorcontrib>Caput, Daniel</creatorcontrib><creatorcontrib>Ferrara, Pascual</creatorcontrib><creatorcontrib>Ruscetti, Francis W.</creatorcontrib><title>IL-4 and IL-13 have overlapping but distinct effects on HIV production in monocytes</title><title>Journal of leukocyte biology</title><addtitle>J Leukoc Biol</addtitle><description>In HIV‐1‐infected monocytes and monocytoid cell lines, viral expression can be observed as high‐level production, restricted (chronic low‐level) expression, and latency (no viral expression). Interleukin‐13 (IL‐13) and IL‐4, which have remarkedly similar deactivating effects on inflammatory monocyte functions, were studied for their regulation of HIV expression in monocytes. Pretreatment of peripheral monocytes for 48–72 h with IL‐13 markedly decreased acute HIV infection, whereas IL‐4 increased it. Similar effects were seen when the U1 and R‐THP‐1 monocytoid cell lines with restricted HIV expression were treated with these cytokines. However, when these continuously producing cell lines were chronically treated with cytokines, IL‐13 increased HIV production. Neither IL‐4 nor IL‐13 stimulated HIV expression in latently infected cells. In chronically infected cells, several cytokines reduced viral mRNA. Both IL‐4 and IL‐13 increased monocyte aggregate formation, but only IL‐4 ultimately stimulated cytolysis of HIV‐infected monocytes as well as increased apoptosis of U1. In the presence of tumor necrosis factor α or IL‐6, which upregulate HIV expression, IL‐13 could no longer suppress HIV expression. These results indicate that IL‐4 and IL‐13, although closely related in modulating monocyte function, can have divergent effects on HIV expression in monocytes. Collectively, these data suggest that there exists a complex cytokine tissue environment with positive regulators of HIV expression able to override negative regulators. J. Leukoc. Biol. 56: 340–346; 1994.</description><subject>Cells, Cultured</subject><subject>cytokines</subject><subject>DNA, Viral - analysis</subject><subject>DNA, Viral - genetics</subject><subject>Gene Expression Regulation, Viral - drug effects</subject><subject>HIV - genetics</subject><subject>HIV - isolation & purification</subject><subject>HIV regulation</subject><subject>human immunodeficiency virus 1</subject><subject>Humans</subject><subject>Interleukin-13</subject><subject>Interleukin-4 - pharmacology</subject><subject>Interleukin-6 - pharmacology</subject><subject>Interleukins - pharmacology</subject><subject>monocytes</subject><subject>Monocytes - chemistry</subject><subject>Monocytes - cytology</subject><subject>Monocytes - microbiology</subject><subject>RNA, Messenger - analysis</subject><subject>RNA, Messenger - genetics</subject><subject>RNA, Viral - analysis</subject><subject>RNA, Viral - genetics</subject><subject>Tumor Necrosis Factor-alpha - pharmacology</subject><issn>0741-5400</issn><issn>1938-3673</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>1994</creationdate><recordtype>article</recordtype><recordid>eNp9kL1PwzAQxS0EKqWwsSJ5gYmUcxw78QgV0KJKDHysluM4rat8lDhp1P8eo1Qdme5O9-690w-hawJTAhA-bIp0yviUTmkEJ2hMBE0CymN6isYQRyRgEcA5unBuAwA05DBCo1gQDhTG6GOxDCKsqgz7hlC8VjuD651pCrXd2mqF067FmXWtrXSLTZ4b3TpcV3i--Mbbps463Vo_2gqXdVXrfWvcJTrLVeHM1aFO0NfL8-dsHizfXxezx2WgaUggEMAhYowTlvFURCSNWZKriIWaJhkTAuKcglBKJ0QILkiWCENFovPE0DTOGZ2gu8HX__HTGdfK0jptikJVpu6cJJxxEUPshfeDUDe1c43J5baxpWr2koD8Yyg9Q8m4pNIz9PKbg2-XliY7ig_Q_J4M-94WZv-vl3xbPsHgeTvcrO1q3dvGSFeqovAJoez7_pj9C31mho8</recordid><startdate>199409</startdate><enddate>199409</enddate><creator>Mikovits, Judy A.</creator><creator>Meyers, Anne M.</creator><creator>Ortaldo, John R.</creator><creator>Minty, Adrian</creator><creator>Caput, Daniel</creator><creator>Ferrara, Pascual</creator><creator>Ruscetti, Francis W.</creator><general>Society for Leukocyte Biology</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7T5</scope><scope>7U9</scope><scope>H94</scope></search><sort><creationdate>199409</creationdate><title>IL-4 and IL-13 have overlapping but distinct effects on HIV production in monocytes</title><author>Mikovits, Judy A. ; Meyers, Anne M. ; Ortaldo, John R. ; Minty, Adrian ; Caput, Daniel ; Ferrara, Pascual ; Ruscetti, Francis W.</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c3210-9060455615d6b941b758fa452c38d59907f309aac8199691d89e398cf8e3b7f53</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>1994</creationdate><topic>Cells, Cultured</topic><topic>cytokines</topic><topic>DNA, Viral - analysis</topic><topic>DNA, Viral - genetics</topic><topic>Gene Expression Regulation, Viral - drug effects</topic><topic>HIV - genetics</topic><topic>HIV - isolation & purification</topic><topic>HIV regulation</topic><topic>human immunodeficiency virus 1</topic><topic>Humans</topic><topic>Interleukin-13</topic><topic>Interleukin-4 - pharmacology</topic><topic>Interleukin-6 - pharmacology</topic><topic>Interleukins - pharmacology</topic><topic>monocytes</topic><topic>Monocytes - chemistry</topic><topic>Monocytes - cytology</topic><topic>Monocytes - microbiology</topic><topic>RNA, Messenger - analysis</topic><topic>RNA, Messenger - genetics</topic><topic>RNA, Viral - analysis</topic><topic>RNA, Viral - genetics</topic><topic>Tumor Necrosis Factor-alpha - pharmacology</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Mikovits, Judy A.</creatorcontrib><creatorcontrib>Meyers, Anne M.</creatorcontrib><creatorcontrib>Ortaldo, John R.</creatorcontrib><creatorcontrib>Minty, Adrian</creatorcontrib><creatorcontrib>Caput, Daniel</creatorcontrib><creatorcontrib>Ferrara, Pascual</creatorcontrib><creatorcontrib>Ruscetti, Francis W.</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>Immunology Abstracts</collection><collection>Virology and AIDS Abstracts</collection><collection>AIDS and Cancer Research Abstracts</collection><jtitle>Journal of leukocyte biology</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Mikovits, Judy A.</au><au>Meyers, Anne M.</au><au>Ortaldo, John R.</au><au>Minty, Adrian</au><au>Caput, Daniel</au><au>Ferrara, Pascual</au><au>Ruscetti, Francis W.</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>IL-4 and IL-13 have overlapping but distinct effects on HIV production in monocytes</atitle><jtitle>Journal of leukocyte biology</jtitle><addtitle>J Leukoc Biol</addtitle><date>1994-09</date><risdate>1994</risdate><volume>56</volume><issue>3</issue><spage>340</spage><epage>346</epage><pages>340-346</pages><issn>0741-5400</issn><eissn>1938-3673</eissn><abstract>In HIV‐1‐infected monocytes and monocytoid cell lines, viral expression can be observed as high‐level production, restricted (chronic low‐level) expression, and latency (no viral expression). Interleukin‐13 (IL‐13) and IL‐4, which have remarkedly similar deactivating effects on inflammatory monocyte functions, were studied for their regulation of HIV expression in monocytes. Pretreatment of peripheral monocytes for 48–72 h with IL‐13 markedly decreased acute HIV infection, whereas IL‐4 increased it. Similar effects were seen when the U1 and R‐THP‐1 monocytoid cell lines with restricted HIV expression were treated with these cytokines. However, when these continuously producing cell lines were chronically treated with cytokines, IL‐13 increased HIV production. Neither IL‐4 nor IL‐13 stimulated HIV expression in latently infected cells. In chronically infected cells, several cytokines reduced viral mRNA. Both IL‐4 and IL‐13 increased monocyte aggregate formation, but only IL‐4 ultimately stimulated cytolysis of HIV‐infected monocytes as well as increased apoptosis of U1. In the presence of tumor necrosis factor α or IL‐6, which upregulate HIV expression, IL‐13 could no longer suppress HIV expression. These results indicate that IL‐4 and IL‐13, although closely related in modulating monocyte function, can have divergent effects on HIV expression in monocytes. Collectively, these data suggest that there exists a complex cytokine tissue environment with positive regulators of HIV expression able to override negative regulators. J. Leukoc. Biol. 56: 340–346; 1994.</abstract><cop>United States</cop><pub>Society for Leukocyte Biology</pub><pmid>7916030</pmid><doi>10.1002/jlb.56.3.340</doi><tpages>7</tpages></addata></record> |
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subjects | Cells, Cultured cytokines DNA, Viral - analysis DNA, Viral - genetics Gene Expression Regulation, Viral - drug effects HIV - genetics HIV - isolation & purification HIV regulation human immunodeficiency virus 1 Humans Interleukin-13 Interleukin-4 - pharmacology Interleukin-6 - pharmacology Interleukins - pharmacology monocytes Monocytes - chemistry Monocytes - cytology Monocytes - microbiology RNA, Messenger - analysis RNA, Messenger - genetics RNA, Viral - analysis RNA, Viral - genetics Tumor Necrosis Factor-alpha - pharmacology |
title | IL-4 and IL-13 have overlapping but distinct effects on HIV production in monocytes |
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