Copepod feeding behavior and the measurement of grazing rates in vivo and in vitro

• Published in: Hydrobiologia 1988-10, Vol.167-168 (1), p.31-41
• Main Author: HEAD, E. J. H
• Format: Article
• Language: English
• Subjects: Biological and medical sciences; Copepoda; Crustacea; Fundamental and applied biological sciences. Psychology; Invertebrates; Marine; Physiology. Development; Pseudocalanus
• ISSN: 0018-8158; 1573-5117
• DOI: 10.1007/BF00026292

In this paper some aspects of the use of the gut fluorescence method for estimating ingestion rates have been examined. One assumption is that gut turnover time in feeding copepods is equal to the gut clearance time in filtered seawater. When arctic Pseudocalanus were pre-fed on Thalassiosira weisflogii, and then given a trace addition of the same C super(14)-labelled culture, or were transferred to filtered seawater, results suggested that this assumption was probably justified. In another experiment in which Pseudocalanus were fed at the same concentration of either melted ice algae, or pelagic under ice algae, there were significant differences in both gut clearance times and gut pigment levels in the two cases. Pigment: biogenic silica ratios in epontic algae were higher than those in faecal pellets produced by Pseudocalanus feeding on the algae, suggesting that pigment destruction was occurring during grazing. In a 28 hr time course experiment ingestion rates determined by rate of disappearance of particulate chlorophyll were higher than those simultaneously determined by the gut fluorescence method, which also supports the idea of pigment destruction in copepods guts. (DBO)