Optimization protocols and improved strategies of cross-linked enzyme aggregates technology: current development and future challenges

Abstract Cross-linked enzyme aggregate (CLEA) technology has been regarded as an effective carrier-free immobilization method. This method is very attractive due to its simplicity and robustness, as well as for the possibility of using the crude enzyme extract and the opportunity to co-immobilize mu...

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Bibliographic Details
Published in:Critical reviews in biotechnology 2015-03, Vol.35 (1), p.15-28
Main Authors: Cui, Jian Dong, Jia, Shi Ru
Format: Article
Language:English
Subjects:
Biocatalysis
Biotechnology - methods
cross-linked enzyme aggregates
Cross-Linking Reagents - chemistry
enzyme immobilization
Enzymes, Immobilized - chemistry
improved strategies
process optimization
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Summary:Abstract Cross-linked enzyme aggregate (CLEA) technology has been regarded as an effective carrier-free immobilization method. This method is very attractive due to its simplicity and robustness, as well as for the possibility of using the crude enzyme extract and the opportunity to co-immobilize multiple different enzymes. The resulting CLEAs generally exhibit high catalyst productivities, improved storage and operational stability and are easy to recycle. Nowadays, although the technology has been applied to various enzymes, some undesirable properties have limited its further application. To overcome these limitations, novel strategies have been developing in recent years. This mini-review focuses on process optimization, new improved strategies and the latest advances on CLEAs technology.
ISSN:0738-8551
1549-7801
DOI:10.3109/07388551.2013.795516