n-3 PUFA productivity in chemostat cultures of microalgae

Eicosapentaenoic (EPA) and docosahexaenoic (DHA) acid productivities from chemostat cultures of an isolate of Isochrysis galbana have been studied. The productivities reached in the interval of dilution rates between 0.0295/h and 0.0355/h were 1.5 mg/l/h for lipids, 300 mu g/l/h for EPA and 130 mu g...

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Bibliographic Details
Published in:Applied microbiology and biotechnology 1993-02, Vol.38 (5), p.599-605
Main Authors: MOLINA GRIMA, E, SANCHEZ PEREZ, J. A, GARCIA CAMACHO, F, GARCIA SANCHEZ, J. L, LOPEZ ALONSO, D
Format: Article
Language:English
Subjects:
Biological and medical sciences
Biotechnology
Fundamental and applied biological sciences. Psychology
Isochrysis galbana
Marine
Methods. Procedures. Technologies
Microbial engineering. Fermentation and microbial culture technology
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Summary:Eicosapentaenoic (EPA) and docosahexaenoic (DHA) acid productivities from chemostat cultures of an isolate of Isochrysis galbana have been studied. The productivities reached in the interval of dilution rates between 0.0295/h and 0.0355/h were 1.5 mg/l/h for lipids, 300 mu g/l/h for EPA and 130 mu g/l/h for DHA. Furthermore, light attenuation by mutual shading, and agitation speed influences on growth and fatty acid composition were analysed. A model relating steady-state dilution rates to internal average light intensity has been proposed, the parameter values of which obtained by non-linear regression were: maximum specific growth rate ( mu sub(max)) = 0.0426/h; the affinity of cells to light (I sub(k)) = 10.92 W/m super(2); the exponent (n) = 5.13.
ISSN:0175-7598
1432-0614
DOI:10.1007/BF00182796