Fed-batch strategy for enhancing cell growth and C-phycocyanin production of Arthrospira (Spirulina) platensis under phototrophic cultivation

•Arthrospira platensis WH879 shows high potential as an C-phycocyanin producer.•The average irradiance affects C-PC content and cell growth rate in an opposite way.•Controlling a lower nitrogen level was more favorable for C-PC accumulation.•Fed-batch cultivation with medium feeding effectively enha...

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Bibliographic Details
Published in:Bioresource technology 2015-03, Vol.180, p.281-287
Main Authors: Xie, Youping, Jin, Yiwen, Zeng, Xianhai, Chen, Jianfeng, Lu, Yinghua, Jing, Keju
Format: Article
Language:English
Subjects:
Arthrospira platensis
Batch Cell Culture Techniques - methods
C-phycocyanin (C-PC)
Fed-batch
Initial biomass concentration
Light
Light intensity
Nitrogen - metabolism
Phototrophic Processes
Phycocyanin - biosynthesis
Spirulina - cytology
Spirulina - growth & development
Spirulina - metabolism
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Summary:•Arthrospira platensis WH879 shows high potential as an C-phycocyanin producer.•The average irradiance affects C-PC content and cell growth rate in an opposite way.•Controlling a lower nitrogen level was more favorable for C-PC accumulation.•Fed-batch cultivation with medium feeding effectively enhanced the C-PC production. The C-phycocyanin generated in blue-green algae Arthrospira platensis is gaining commercial interest due to its nutrition and healthcare value. In this study, the light intensity and initial biomass concentration were manipulated to improve cell growth and C-phycocyanin production of A.platensis in batch cultivation. The results show that low light intensity and high initial biomass concentration led to increased C-phycocyanin accumulation. The best C-phycocyanin productivity occurred when light intensity and initial biomass concentration were 300μmol/m2/s and 0.24g/L, respectively. The fed-batch cultivation proved to be an effective strategy to further enhance C-phycocyanin production of A.platensis. The results indicate that C-phycocyanin accumulation not only requires nitrogen-sufficient condition, but also needs other nutrients. The highest C-phycocyanin content (16.1%), production (1034mg/L) and productivity (94.8mg/L/d) were obtained when using fed-batch strategy with 5mM medium feeding.
ISSN:0960-8524
1873-2976
DOI:10.1016/j.biortech.2014.12.073