Single pyruvate intake induces blood alkalization and modification of resting metabolism in humans

• Published in: Nutrition (Burbank, Los Angeles County, Calif.) Los Angeles County, Calif.), 2015-03, Vol.31 (3), p.466-474
• Main Authors: Olek, Robert A., Ph.D, Luszczyk, Marcin, Ph.D, Kujach, Sylwester, M.Sc, Ziemann, Ewa, Ph.D, Pieszko, Magdalena, Ph.D, Pischel, Ivo, Ph.D, Laskowski, Radoslaw, Ph.D
• Format: Article
• Language: English
• Subjects: Acid-Base Equilibrium - drug effects; Acid-base status; Adult; Age; Basal Metabolism - drug effects; Base excess; Blood; Blood bicarbonate; Blood Glucose - metabolism; Body fat; Calcium; Carbohydrate Metabolism - drug effects; Dietary supplements; Energy Metabolism; Experiments; Fatty Acids, Nonesterified - blood; Female; Football; Free fatty acids; Gastroenterology and Hepatology; Glycerol; Glycerol - blood; Humans; Hydrogen-Ion Concentration; Ingestion; Laboratories; Lipolysis - drug effects; Male; Metabolism; Nutrition research; Oxygen Consumption; Plasma; Pyruvates - pharmacology; Respiratory exchange ratio; Rest - physiology; Resting energy expenditure; Rodents; Sex Factors; Sodium; Studies; Variance analysis; Weight control; Young Adult
• ISSN: 0899-9007; 1873-1244
• DOI: 10.1016/j.nut.2014.09.012

Abstract Objectives Three separate studies were performed with the aim to 1) determine the effect of a single sodium pyruvate intake on the blood acid-base status in males and females; 2) compare the effect of sodium and calcium pyruvate salts and establish their role in the lipolysis rate; and 3) quantify the effect of single pyruvate intake on the resting energy metabolism. Methods In all, 48 individuals completed three separate studies. In all the studies, participants consumed a single dose of pyruvate 0.1 g/kg 60 min before commencing the measurements. The whole blood pH, bicarbonate concentration, base excess or plasma glycerol, free fatty acids, glucose concentrations, or resting energy expenditure and calculated respiratory exchange ratio were determined. The analysis of variance for repeated measurements was performed to examine the interaction between treatment and time. Results The single dose of sodium pyruvate induced blood alkalization, which was more marked in the male than in the female participants. Following the ingestion of sodium or calcium pyruvate, the blood acid-base parameters were higher than in the placebo trial. Furthermore, 3-h postingestion glycerol was lower in both pyruvate trials than in placebo. Resting energy expenditure did not differ between the trials; however, carbohydrate oxidation was increased after sodium pyruvate ingestion. Conclusion Pyruvate intake induced mild alkalization in a sex-dependent fashion. Moreover, it accelerated carbohydrate metabolism and delayed the rate of glycerol appearance in the blood, but had no effect on the resting energy expenditure. Furthermore, sodium salt seems to have had a greater effect on the blood buffering level than calcium salt.