Surface coating. No improvement of shear stress resistance of anchorage dependent mammalian cells

The growth of adherent Baby Hamster Kidney cells (BHK 21) on surfaces coated with different substances (FCS, Con. A, PHA, cholesterol, collagen) was investigated. A positive influence on cell viability could only be observed when using a fetal calf serum (FCS) coat. To evaluate the effects of coatin...

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Bibliographic Details
Published in:Biotechnology techniques 1993-07, Vol.7 (7), p.411-416
Main Authors: LUDWIG, A, WEIDEMANN, R, TOMECZKOWSKI, J, KRETZMER, G
Format: Article
Language:English
Subjects:
Animal cells
Biological and medical sciences
Biotechnology
Eukaryotic cell cultures
Fundamental and applied biological sciences. Psychology
hamster
Methods. Procedures. Technologies
Miscellaneous
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Summary:The growth of adherent Baby Hamster Kidney cells (BHK 21) on surfaces coated with different substances (FCS, Con. A, PHA, cholesterol, collagen) was investigated. A positive influence on cell viability could only be observed when using a fetal calf serum (FCS) coat. To evaluate the effects of coating on shear stress sensitivity, experiments in a home made flow chamber were carried out. For comparison further investigations were conducted for several serum concentrations in the medium. The results demonstrate that the latter had a more beneficial effect on shear stress resistance than coating.
ISSN:0951-208X
1573-6784
DOI:10.1007/BF00151875