Mannose fermentation by an ethanologenic recombinant Escherichia coli

Recombinant E. coli B (pLOI297) grows in Luria broth with mannose at a rate that is only about one-half of the rate with xylose and about one-quarter of the rate with glucose as carbon source. For a sugar concentration of about 2% (w/v), the corresponding specific ethanol productivities (qp) are 0.2...

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Bibliographic Details
Published in:Biotechnology letters 1993-06, Vol.15 (6), p.615-620
Main Authors: Lawford, H.G, Rousseau, J.D
Format: Article
Language:English
Subjects:
Biological and medical sciences
Biotechnology
Escherichia coli
ethanol production
fermentation
Fundamental and applied biological sciences. Psychology
genetic recombination
glucose
growth
hemicellulose
mannose
Methods. Procedures. Technologies
Microbial engineering. Fermentation and microbial culture technology
productivity
synthetic softwood prehydrolysate
xylose
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Summary:Recombinant E. coli B (pLOI297) grows in Luria broth with mannose at a rate that is only about one-half of the rate with xylose and about one-quarter of the rate with glucose as carbon source. For a sugar concentration of about 2% (w/v), the corresponding specific ethanol productivities (qp) are 0.22, 0.45 and 0.70 g ethanol/g cell/h for mannose, xylose and glucose. At higher sugar concentrations (8-11%), the sp. productivities are 0.12, 0.33 and 0.35 g ethanol/g cell/h for mannose, xylose and glucose. Using a synthetic softwood prehydrolysate medium, in which the mass ratio of mannose:xylose:glucose was approx. 1.0:0.6:0.4 (total sugar conc'n 4.5%), the sp. productivities associated with glucose and xylose metabolism were, decreased by about 50% and 75% respectively, whereas mannose metabolism appeared unaffected by the presence of the other sugars. In all cases, the sugar-to-ethanol conversion efficiency was > 90% of theoretical maximum.
ISSN:0141-5492
1573-6776
DOI:10.1007/BF00138551