Electron transfer reactions for the reduction of NADP super(+) in Methanosphaera stadtmanae

Methanosphaera stadtmanae, a member of the Methanobacteriales reduces methanol, but not CO sub(2) with H sub(2) or 2-propanol to produce methane. In cell-free extracts of M. stadtmanae the activities of several enzymes involved in electron transfer were measured. The activities of an F sub(420)-nonr...

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Bibliographic Details
Published in:FEMS microbiology letters 1994-01, Vol.120 (3), p.285-290
Main Authors: Wong, D, Lin, Z, Juck, D F, Terrick, KA, Sparling, R
Format: Article
Language:English
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Summary:Methanosphaera stadtmanae, a member of the Methanobacteriales reduces methanol, but not CO sub(2) with H sub(2) or 2-propanol to produce methane. In cell-free extracts of M. stadtmanae the activities of several enzymes involved in electron transfer were measured. The activities of an F sub(420)-nonreactive hydrogenase, NADP super(+):F sub(420) oxidoreductase, NADP super(+)-dependent 2-propanol dehydrogenase, and a methyl viologen dependent F sub(420) dehydrogenase were observed. Based on the presence of these particular enzyme activities, their cofactor requirements and the absence of F sub(420)-dependent hydrogenase activity, a model of the electron transport pathway through the coenzyme F sub(420) to provide electrons for biosynthesis, was formulated.
ISSN:0378-1097