Cyclooxygenase is an immediate-early gene induced by interleukin-1 in human endothelial cells

The monokine interleukin-1 (IL-1) inhibits endothelial cell growth and induces prostacyclin production in human endothelial cells. Since cyclooxygenase (Cox) is the rate-limiting enzyme in the synthesis of prostanoids, we evaluated the ability of IL-1 to stimulate Cox expression by human umbilical v...

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Bibliographic Details
Published in:The Journal of biological chemistry 1990-07, Vol.265 (19), p.10805-10808
Main Authors: MAIER, J. A. M, HLA, T, MACIAG, T
Format: Article
Language:English
Subjects:
Analytical, structural and metabolic biochemistry
Biological and medical sciences
Blotting, Western
Cycloheximide - pharmacology
Dactinomycin - pharmacology
Drug Synergism
Endothelium, Vascular - enzymology
Enzymes and enzyme inhibitors
Fundamental and applied biological sciences. Psychology
Gene Expression - drug effects
Humans
Interleukin-1 - pharmacology
Kinetics
Nucleic Acid Hybridization
Oxidoreductases
Polymerase Chain Reaction
Prostaglandin-Endoperoxide Synthases - genetics
Prostaglandins - biosynthesis
RNA, Messenger - biosynthesis
RNA-Directed DNA Polymerase
Transcription, Genetic
Umbilical Veins
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Summary:The monokine interleukin-1 (IL-1) inhibits endothelial cell growth and induces prostacyclin production in human endothelial cells. Since cyclooxygenase (Cox) is the rate-limiting enzyme in the synthesis of prostanoids, we evaluated the ability of IL-1 to stimulate Cox expression by human umbilical vein endothelial cells (HUVEC) in vitro. Our data demonstrate that 1) the Cox mRNA is expressed at low levels in untreated cells; 2) IL-1 alpha induces the Cox mRNA within 2 h, and this induction is sustained for more than 24 h; 3) IL-1 alpha induction is dose-dependent; 4) cycloheximide potentiates the induction of the Cox mRNA by IL-1 alpha while actinomycin D prevents the induction, and 5) IL-1 alpha also stimulates Cox production in a time-dependent fashion which correlates with the increase in prostacyclin synthesis. These data suggest that Cox is an immediate-early gene induced by IL-1 in HUVEC and may contribute to the regulation of the endothelial cell differentiation pathway in vitro.
ISSN:0021-9258
1083-351X
DOI:10.1016/s0021-9258(19)38515-1