Biochemical studies on the lethal effects of solar and artificial ultraviolet radiation on Staphylococcus aureus

The effects of UV-B radiation generated in the laboratory and as a component of sunlight on the viability and particular biochemical activities of the bacterium Staphylococcus aureus have been examined. UV-B radiation progressively inhibits protein synthesis (assayed as 3H-alanine incorporation) and...

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Bibliographic Details
Published in:Archives of microbiology 1994, Vol.161 (1), p.82-87
Main Authors: EL-ADHAMI, W, DALY, S, STEWART, P. R
Format: Article
Language:English
Subjects:
Action of physical and chemical agents on bacteria
Bacteriology
Biological and medical sciences
Chelating Agents
DNA Replication - radiation effects
Dose-Response Relationship, Radiation
Ferric Compounds
Free Radical Scavengers
Free Radicals
Fundamental and applied biological sciences. Psychology
Light
Microbiology
Oxygen - chemistry
Oxygen Consumption - radiation effects
Protein Biosynthesis - radiation effects
Staphylococcus aureus - radiation effects
Ultraviolet Rays
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Summary:The effects of UV-B radiation generated in the laboratory and as a component of sunlight on the viability and particular biochemical activities of the bacterium Staphylococcus aureus have been examined. UV-B radiation progressively inhibits protein synthesis (assayed as 3H-alanine incorporation) and kills cells. Cell respiration, and RNA and DNA synthesis (3H-uridine and 3H-thymidine incorporation) were not greatly affected by UV-B irradiation. The OH. and 1O2-free radical scavengers protected cells against killing and inhibition of protein synthesis by UV-B, suggesting that such radicals mediate the effects of UV-B on this organism. A similar protective effect using a ferric ion chelator suggests an important role for metallic ions in UV-B lethality.
ISSN:0302-8933
1432-072X
DOI:10.1007/BF00248897