Large‐scale analysis of viral nucleic acid spectrum in temporal lobe epilepsy biopsies

Summary Objective Chronic inflammatory processes are important promotors of temporal lobe epilepsy (TLE) development. Based on human herpesvirus 6 (HHV‐6) DNA detection in brain tissue from patients with TLE, an association of persistent viral infection with TLE has been discussed. Individual studie...

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Published in:Epilepsia (Copenhagen) 2015-02, Vol.56 (2), p.234-243
Main Authors: Esposito, Laura, Drexler, Jan F., Braganza, Oliver, Doberentz, Elke, Grote, Alexander, Widman, Guido, Drosten, Christian, Eis‐Hübinger, Anna M., Schoch, Susanne, Elger, Christian E., Becker, Albert J., Niehusmann, Pitt
Format: Article
Language:English
Subjects:
Adolescent
Adult
Aged
Biopsy
DNA, Viral - analysis
Encephalitis
Epilepsy, Temporal Lobe - pathology
Epilepsy, Temporal Lobe - virology
Epileptogenesis
Febrile seizures
Female
Herpesvirus 6, Human - genetics
HHV‐6
Humans
Inflammation
Male
Middle Aged
Polymerase Chain Reaction - methods
Young Adult
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Summary:Summary Objective Chronic inflammatory processes are important promotors of temporal lobe epilepsy (TLE) development. Based on human herpesvirus 6 (HHV‐6) DNA detection in brain tissue from patients with TLE, an association of persistent viral infection with TLE has been discussed. Individual studies reported increased HHV‐6 DNA in patients with clinical signs of previous inflammatory brain reaction, that is, febrile seizures or meningoencephalitis. However, detection rates vary considerably between different studies. Here we performed a large‐scale analysis of viral DNA/RNA spectrum in high‐quality TLE biopsies. In addition to all Herpesviridae, we addressed potentially relevant neurotropic RNA viruses. Methods DNA and RNA were extracted from 346 fresh‐frozen tissue samples removed by epilepsy surgery. Real‐time polymerase chain reaction (PCR) and nested PCR were performed for Herpesviridae and RNA viruses, respectively. Clinical data were analyzed for earlier signs of inflammatory brain reactions. Fresh‐frozen hippocampal tissue samples from patients without chronic central nervous system (CNS) disease served as controls (n = 62). Seven previous PCR studies with overall 178 TLE patients were additionally analyzed regarding a correlation of clinical parameters and HHV‐6 detection. Results PCR revealed HHV‐6B DNA in 34 specimens (9.8%) from TLE patients. HHV‐6B DNA was also present in eight control samples (12.9%; p > 0.05), but showed a lower virus concentration (p 
ISSN:0013-9580
1528-1167
DOI:10.1111/epi.12890