Live Eyeworm (Oxyspirura petrowi) Extraction, In Vitro Culture, and Transfer for Experimental Studies

Northern bobwhite (Colinus virginianus) have experienced a dramatic decline in West Texas over the last 3 yr, and investigations are underway to evaluate the role of parasites in this decline. One of the key parasites being investigated is the eyeworm (Oxyspirura petrowi). Live eyeworms were extract...

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Bibliographic Details
Published in:The Journal of parasitology 2015-02, Vol.101 (1), p.98-101
Main Authors: Dunham, N. R, Soliz, L. A, Brightman, A, Rollins, D, Fedynich, A. M, Kendall, R. J
Format: Article
Language:English
Subjects:
Animals
Bird Diseases - parasitology
Colinus - parasitology
Colinus virginianus
Culture Media
egg albumen
Eye - parasitology
Eye - pathology
Eye Infections, Parasitic - parasitology
Eye Infections, Parasitic - veterinary
eyes
Female
hosts
life history
Male
Movement
Oxyspirura
parasites
Sexual Behavior, Animal
SHORT COMMUNICATIONS
sodium chloride
Spirurida Infections - parasitology
Spirurida Infections - veterinary
Thelazioidea - growth & development
Thelazioidea - isolation & purification
Thelazioidea - physiology
Time Factors
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Summary:Northern bobwhite (Colinus virginianus) have experienced a dramatic decline in West Texas over the last 3 yr, and investigations are underway to evaluate the role of parasites in this decline. One of the key parasites being investigated is the eyeworm (Oxyspirura petrowi). Live eyeworms were extracted from both live and dead northern bobwhites, and in vitro survival was tested using 10 liquid media. Eyeworms placed in an egg white and physiological saline solution lived for at least 36 days. Live O. petrowi placed into the eyes of uninfected pen-raised bobwhites were monitored for 21 days to demonstrate successful transfer. Eyeworm behavior during feeding, mating, and development were monitored. This study is important to research that requires “banking” of live O. petrowi from wild-captured definitive hosts for life history studies and assessing the impact of O. petrowi on host individuals.
ISSN:0022-3395
1937-2345
DOI:10.1645/13-448.1