A simple dynamic method for on-line and off-line determination of k sub(L)a during cultivation of animal cells

A new, fast method is described to determine k sub(L)a either off-line, or on-line during animal-cell cultivation. Since it does not need the equilibrium concentration of oxygen in the liquid phase (C*), it is not required to await a new steady state. Furthermore, the results do not depend on the ca...

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Bibliographic Details
Published in:Biotechnology techniques 1994-01, Vol.8 (9), p.675-680
Main Authors: Dorresteijn, R C, De Gooijer, CD, Tramper, J, Beuvery, E C
Format: Article
Language:English
Online Access:Get full text
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Summary:A new, fast method is described to determine k sub(L)a either off-line, or on-line during animal-cell cultivation. Since it does not need the equilibrium concentration of oxygen in the liquid phase (C*), it is not required to await a new steady state. Furthermore, the results do not depend on the calibration value of the dissolved-oxygen probe. The method yielded accurate values for k sub(L)a, both for an oxygen-consuming and a non-consuming system.
ISSN:0951-208X