Human chromosomal centromere (AATGG) sub(n) sequence forms stable structures with unusual base pairs

Nine DNA sequences related to the purine strand of the human centromeric satellite (AATGG) sub(n)-(CCATT) sub(n) repeat have been studied by two-dimensional nuclear magnetic resonance spectroscopy. Earlier studies have suggested that the structure of (AATGG) sub(n) sequence has an equilibrium betwee...

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Bibliographic Details
Published in:FEBS letters 1994-01, Vol.347 (1), p.99-103
Main Authors: Jaishree, T N, Wang, AH-J
Format: Article
Language:English
Online Access:Get full text
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Summary:Nine DNA sequences related to the purine strand of the human centromeric satellite (AATGG) sub(n)-(CCATT) sub(n) repeat have been studied by two-dimensional nuclear magnetic resonance spectroscopy. Earlier studies have suggested that the structure of (AATGG) sub(n) sequence has an equilibrium between the duplex form and a fold-back form. Structural refinement of d(CAATGG) and its related sequences by an NOE-constrained simulated annealing procedure reveals that the duplex form incorporates dynamic type-I G-A base pairs. 1D exchangeable proton NMR data support this model. The reverse sequence motif (GGTAA) destabilizes the structure.
ISSN:0014-5793