A flexible microneedle array as low-voltage electroporation electrodes for in vivoDNA and siRNA delivery

In vivoelectroporation is an appealing method to deliver nucleic acid into living tissues, but the clinical application of such a method was limited due to severe tissue damage and poor coverage of the tissue surface. Here we present the validation of a novel flexible microneedle array electrode (MN...

Full description

Saved in:
Bibliographic Details
Published in:Lab on a chip 2014-09, Vol.14 (20), p.4093-4102
Main Authors: Wei, Zewen, Zheng, Shuquan, Wang, Renxin, Bu, Xiangli, Ma, Huailei, Wu, Yidi, Zhu, Ling, Hu, Zhiyuan, Liang, Zicai, Li, Zhihong
Format: Article
Language:English
Subjects:
Arrays
Chips
Damage
Deoxyribonucleic acid
Electric potential
Electrodes
Electroporation
Voltage
Online Access:Get full text
Tags: Add Tag
No Tags, Be the first to tag this record!
Description
Summary:In vivoelectroporation is an appealing method to deliver nucleic acid into living tissues, but the clinical application of such a method was limited due to severe tissue damage and poor coverage of the tissue surface. Here we present the validation of a novel flexible microneedle array electrode (MNAE) chip, in which the microneedle array and the flexible substrate are integrated together to simultaneously facilitate low-voltage electroporation and accomplish good coverage of the tissue surface. The efficient delivery of both DNA and siRNA was demonstrated on mice. Upon penetrating the high-resistance stratum corneum, the electroporation voltage was reduced to about 35 V, which was generally recognized safe for humans. Also, a pathological analysis of the microneedle-electroporated tissues was carried out to thoroughly assess the skin damage, which is an important consideration in pre-clinical studies of electroporation devices. This MNAE constitutes a novel way of in vivodelivery of siRNA and DNA to certain tissues or organs with satisfactory efficiency and good adaptation to the tissue surface profile as well as minimum tissue damage, thus avoiding the disadvantages of existing electroporation methods.
ISSN:1473-0197
1473-0189
DOI:10.1039/c4lc00800f