Sensitive determination of atorvastatin in human plasma by dispersive liquid-liquid microextraction and solidification of floating organic drop followed by high-performance liquid chromatography

A novel and sensitive dispersive liquid–liquid microextraction method based on the solidification of the floating organic drop combined with high‐performance liquid chromatography and ultraviolet detection was used for the determination of atorvastatine in blood serum samples. The chromatographic se...

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Bibliographic Details
Published in:Journal of separation science 2015-01, Vol.38 (2), p.309-315
Main Authors: Taheri, Salman, Jalali, Fahimeh, Fattahi, Nazir, Bahrami, Gholamreza
Format: Article
Language:English
Subjects:
Adult
Atorvastatin
Atorvastatin Calcium
Blood analysis
Calibration
Chromatography
Chromatography, High Pressure Liquid - methods
Female
Heptanoic Acids - blood
Human
Humans
Hydrogen-Ion Concentration
Hydroxymethylglutaryl-CoA Reductase Inhibitors - blood
Limit of Detection
Liquid chromatography
Liquid Phase Microextraction - methods
Liquid-liquid extraction
Male
Methyl alcohol
Microextraction
Organic drop
Plasma
Pyrroles - blood
Separation
Sodium Chloride - chemistry
Solidification
Solvents
Spectrophotometry, Ultraviolet
Statins
Young Adult
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Summary:A novel and sensitive dispersive liquid–liquid microextraction method based on the solidification of the floating organic drop combined with high‐performance liquid chromatography and ultraviolet detection was used for the determination of atorvastatine in blood serum samples. The chromatographic separation of atorvastatin was carried out using methanol as the mobile phase organic modifier. Various parameters affecting the extraction efficiency were optimized, such as the kind and volume of extraction solvent (1‐undecanol) and disperser solvent (acetonitrile), pH, and the extraction time. The calibration curve was linear in the range of 0.2–6000 μg/L of atorvastatin (r2 = 0.995) with a limit of detection of 0.07 μg/L. The relative standard deviation for 100 μg/L of atorvastatin in human plasma was 8.4% (n = 4). The recoveries of plasma samples spiked with atorvastatin were in the range of 98.8–113.8%. The obtained results showed that the proposed method is fast, simple, and reliable for the determination of very low concentrations of atorvastatin in human plasma samples.
ISSN:1615-9306
1615-9314
DOI:10.1002/jssc.201401115