Development of a multiplex flow-through immunoaffinity chromatography test for the on-site screening of 14 sulfonamide and 13 quinolone residues in milk

In this paper, a rapid and sensitive multiplex flow-through immunoaffinity chromatography test (FTIACT) was developed for the on-site screening of 14 sulfonamide and 13 quinolone residues in milk. The developed FTIACT method combines the purification, preconcentration and immunochemical detection of...

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Bibliographic Details
Published in:Biosensors & bioelectronics 2015-04, Vol.66, p.124-128
Main Authors: Jiang, Wenxiao, Beloglazova, Natalia V., Wang, Zhanhui, Jiang, Haiyang, Wen, Kai, de Saeger, Sarah, Luo, Pengjie, Wu, Yongning, Shen, Jianzhong
Format: Article
Language:English
Subjects:
Animals
Bioelectricity
Biosensors
Chromatography
Food Contamination - analysis
Food safety
Image detection
Immunoassay
Immunochromatography - instrumentation
Immunochromatography - methods
Limit of Detection
Milk
Milk - chemistry
Multiplexing
Quantitative analysis
Quinolones
Quinolones - analysis
Residues
Screening
Sulfonamides
Sulfonamides - analysis
Veterinary drug residue
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Summary:In this paper, a rapid and sensitive multiplex flow-through immunoaffinity chromatography test (FTIACT) was developed for the on-site screening of 14 sulfonamide and 13 quinolone residues in milk. The developed FTIACT method combines the purification, preconcentration and immunochemical detection of multiple antibiotics on the sepharose gel test layers. The use of liposome-encapsulated quantum dots (LQDs) with the FTIACT method exhibited the best results, with limits of detection (LODs) of 1 and 0.5ng/mL for the sulfonamides (SAs) and quinolones (QNs), respectively, through qualitative analysis (visual detection by the naked eye). In order to achieve low detection limit, the color intensity of the images were converted into relative optical density values to enable a quantitative evaluation. Quantitative analysis of the samples enabled the detection of SAs (0.13ng/mL) and QNs (0.062ng/mL) in spiked milk samples. The FTIACT described in this work shows promise as a multiplex immunoassay for the qualitative and quantitative screening of multiple chemical residues in milk. •A multiplex FTIACT was developed for screening of 14 SAs and 13 QNs residues in milk.•The FTIACT could qualitative and quantitative detect SAs and QNs residues in milk.•The FTIACT shows promise as a multiplex immunoassay for multiple chemical residues analysis.
ISSN:0956-5663
1873-4235
DOI:10.1016/j.bios.2014.11.004