Cloud point extraction of ?9-tetrahydrocannabinol from cannabis resin

A cloud point extraction coupled with high performance liquid chromatography (HPLC/UV) method was developed for the determination of ?9-tetrahydrocannabinol (THC) in micellar phase. The nonionic surfactant aDowfax 20B102a was used to extract and pre-concentrate THC from cannabis resin, prior to its...

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Bibliographic Details
Published in:Analytical and bioanalytical chemistry 2013-04, Vol.405 (10), p.3117-3123
Main Authors: Ameur, S, Haddou, B, Derriche, Z, Canselier, J P, Gourdon, C
Format: Article
Language:English
Subjects:
Arrays
Cannabis
Diodes
Extraction
Mathematical analysis
Polymers
Resins
Standard deviation
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Summary:A cloud point extraction coupled with high performance liquid chromatography (HPLC/UV) method was developed for the determination of ?9-tetrahydrocannabinol (THC) in micellar phase. The nonionic surfactant aDowfax 20B102a was used to extract and pre-concentrate THC from cannabis resin, prior to its determination with a HPLCaUV system (diode array detector) with isocratic elution. The parameters and variables affecting the extraction were investigated. Under optimum conditions (1 wt.% Dowfax 20B102, 1 wt.% Na2SO4, T?=?318 K, t?=?30 min), this method yielded a quite satisfactory recovery rate (~81 %). The limit of detection was 0.04 ?g?mL?1, and the relative standard deviation was less than 2 %. Compared with conventional solid-liquid extraction, this new method avoids the use of volatile organic solvents, therefore is environmentally safer.
ISSN:1618-2642
1618-2650
DOI:10.1007/s00216-013-6743-2