A whole-cell bioreporter approach for the genotoxicity assessment of bioavailability of toxic compounds in contaminated soil in China

A whole-cell bacterial bioreporter Acinetobacter baylyi strain ADP1_recA_lux that responds to genotoxins was employed to directly assess the adverse effects of the bioavailable fraction of mitomycin C (MMC), benzo[a]pyrene (BaP), chromium (VI) and lead (II) in amended soils and soil samples from two...

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Bibliographic Details
Published in:Environmental pollution (1987) 2014-12, Vol.195, p.178-184
Main Authors: YIZHI SONG, BO JIANG, SICONG TIAN, HUI TANG, ZENGJUN LIU, CHUAN LI, JIANLI JIA, HUANG, Wei E, XU ZHANG, GUANGHE LI
Format: Article
Language:English
Subjects:
Acinetobacter
Bacteria
Benzo(a)pyrene - analysis
Benzo(a)pyrene - toxicity
Biological and medical sciences
Biological Assay
China
Chromium
Chromium - analysis
Chromium - toxicity
DNA Damage
Environmental Monitoring - methods
Environmental pollutants toxicology
Environmental Pollution - statistics & numerical data
General aspects
Genotoxicity
Hazardous Substances - analysis
Hazardous Substances - toxicity
Lead (metal)
Medical sciences
Metal matrix composites
Mutagens - analysis
Mutagens - toxicity
Pollutants
Soil (material)
Soil - chemistry
Soil Pollutants - analysis
Soil Pollutants - toxicity
Toxicology
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Summary:A whole-cell bacterial bioreporter Acinetobacter baylyi strain ADP1_recA_lux that responds to genotoxins was employed to directly assess the adverse effects of the bioavailable fraction of mitomycin C (MMC), benzo[a]pyrene (BaP), chromium (VI) and lead (II) in amended soils and soil samples from two fragile areas in China without soil pre-treatment. The amended soils containing pollutants with the concentrations as low as 0.4 mg/kg MMC, 0.5 mg/kg BaP, 520 mg/kg Cr (VI) and 2072 mg/kg Pb (II) were found to be toxic. Soil particle-associated pollutants accounted for 86%, 100%, 29%, and 92% of the genotoxicity in the MMC, BaP, Cr (VI), and Pb (II) amended soil, respectively. The soils from contaminated sites were also valid to be genotoxic. The results suggest both free and soil particle-associated pollutants are bioavailable to soil organisms and a solid-phase contact bioreporter assay to soil contamination could provide a rapid screening tool for environmental risk assessment.
ISSN:0269-7491
1873-6424
DOI:10.1016/j.envpol.2014.08.024