Effects of red laser, infrared, photodynamic therapy, and green LED on the healing process of third-degree burns: clinical and histological study in rats

The aim of this study was to evaluate the effects of red laser, infrared, photodynamic therapy, and green light-emitting diode (LED) on the healing process of skin burns through clinical and histopathologic analysis in rats. For this, 100 animals were randomly divided into five groups: G1—untreated...

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Bibliographic Details
Published in:Lasers in medical science 2015-01, Vol.30 (1), p.421-428
Main Authors: de Vasconcelos Catão, Maria Helena Chaves, Nonaka, Cassiano Francisco Weege, de Albuquerque, Ricardo Luiz Cavalcanti, Bento, Patrícia Meira, de Oliveira Costa, Roniery
Format: Article
Language:English
Subjects:
Animals
Burn treatment
Burns
Burns - drug therapy
Burns - pathology
Burns - radiotherapy
Collagen - metabolism
Dentistry
Drinking Behavior
Feeding Behavior
Healing
Histology
Inflammation - pathology
Infrared
Infrared Rays
Lasers
Light emitting diodes
Male
Medicine
Medicine & Public Health
Optical Devices
Optics
Optics and Photonics
Original Article
Photochemotherapy
Photodynamic therapy
Photonics
Photosensitizing Agents - therapeutic use
Quantum Optics
Rats
Rats, Wistar
Rodents
Wound healing
Wound Healing - radiation effects
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Summary:The aim of this study was to evaluate the effects of red laser, infrared, photodynamic therapy, and green light-emitting diode (LED) on the healing process of skin burns through clinical and histopathologic analysis in rats. For this, 100 animals were randomly divided into five groups: G1—untreated control (CTR), G2—red laser (LVER), G3—infrared (LINF), G4—photodynamic therapy (PDT), and G5—green LED. Burn was induced on the dorsum of the rat and the treatment of the experimental groups was red light (10 J/cm 2 , 10 s, 40 mW, and λ660 nm), infrared (10 J/cm 2 , 10 s, 40 mW, and λ780 nm), green LED irradiation (60 J/cm 2 , 10 s, λ520, and 550 nm), and photodynamic therapy (10 J/cm 2 , 40 mW, and λ660 nm), the latter combined with methylene blue photosensitizer at concentration 0.5 μg/mL. Applications were performed daily until day prior to sacrifice of the animal at 3, 7, 14, and 21 days with intraperitoneal anesthetic overdose. The specimens collected were clinically examined and soon after processed and stained with hematoxylin-eosin and Picrosirius for analysis under light and polarized light microscopy, respectively. Animals treated with LVER, LINF, PDT ( p  
ISSN:0268-8921
1435-604X
DOI:10.1007/s10103-014-1687-0