Mass spectrometry based phospholipidomics of mammalian thymus and leukemia patients: implication for function of iNKT cells

• Published in: Analytical and bioanalytical chemistry 2013-06, Vol.405 (15), p.5267-5278
• Main Authors: Xu, Xiukun, Yu, Yunhui, Wang, Zheng, Zhu, Tingting, Wang, Yanping, Zhu, Jian, Chen, Zijun, He, Yun, Ju, Linling, Li, Yunsen
• Format: Article
• Language: English
• Subjects: Analytical Chemistry; Animals; Antigens; Biochemistry; Bone marrow; Bone Marrow Cells - metabolism; Cancer; Characterization and Evaluation of Materials; Chemistry; Chemistry and Materials Science; Chromatography; Food Science; Gene Expression Regulation; Health aspects; Humans; Ionization; Laboratory Medicine; Leukemia; Leukemia - metabolism; Leukemias; Ligands; Lipids; Liver; Mammals; Mass spectrometry; Mass Spectrometry - methods; Metabolites; Metabolomics; Methods; Mice; Monitoring/Environmental Analysis; Natural Killer T-Cells - metabolism; Patients; Phospholipids; Phospholipids - chemistry; Phospholipids - metabolism; Physiological aspects; Proteomics; Research Paper; Scientific imaging; Spleen; Thymus gland; Thymus Gland - metabolism
• ISSN: 1618-2642; 1618-2650
• DOI: 10.1007/s00216-013-6923-0

In previous studies phospholipids have been proved to be involved in biochemical, physiological, and pathological processes. As a special class of phospholipids, peroxisome-derived lipids (PDLs) have been proved to be potential ligands of invariant natural killer T (iNKT) cells in recent studies. Here, on the basis of phospholipidomics, we focused on the relative quantity of PDLs extracted from mammalian thymus or bone marrow using electrospray ionization mass spectrometry (MS). In phospholipid analysis, we identified 12 classes of phospholipids and accounted for their relative quantities by comparing their relative abundances in the MS 1 map. Our results show that PDLs are present in mammalian thymus as well as mouse spleen and liver. Interestingly, the relative quantity of PDLs extracted from human acute myeloid leukemia (AML) and acute lymphoblastic leukemia (ALL) bone marrows is higher than that extracted from bone marrow of healthy donors. Our results may help to explain the close correlation between PDLs and iNKT cell function in thymus, spleen, liver, and especially in leukemia patients. We think that our phospholipidomics work may reveal a function of iNKT cells.