Rapid and sensitive HILIC–MS/MS analysis of carnitine and acetylcarnitine in biological fluids

Monitoring carnitine and acetylcarnitine levels in biological fluids is a powerful tool for diagnostic studies. Research has recently shown that the analysis of carnitine and related compounds in clinical samples can be accomplished by different analytical approaches. Because of the polar and ionic...

Full description

Saved in:
Bibliographic Details
Published in:Analytical and bioanalytical chemistry 2013-09, Vol.405 (23), p.7397-7404
Main Authors: Isaguirre, Andrea C., Olsina, Roberto A., Martinez, Luis D., Lapierre, Alicia V., Cerutti, Soledad
Format: Article
Language:English
Subjects:
Acetylcarnitine - blood
Acetylcarnitine - urine
Adult
Analytical Chemistry
Biochemistry
Biological
Biological products
Carnitine
Carnitine - blood
Carnitine - urine
Characterization and Evaluation of Materials
Chemical properties
Chemistry
Chemistry and Materials Science
Chromatography
Chromatography, Liquid
Composition
Fatty acids
Female
Flame retardants
Fluid dynamics
Fluid flow
Fluids
Food Science
Humans
Hydrophobic and Hydrophilic Interactions
Identification and classification
Ionization
Laboratory Medicine
Limit of Detection
Liquid chromatography
Male
Mass spectrometry
Mathematical analysis
Metabolism
Metabolites
Methods
Middle Aged
Monitoring/Environmental Analysis
Nanostructure
Oxidation
Research Paper
Retention
Scientific imaging
Tandem Mass Spectrometry
Citations: Items that this one cites
Items that cite this one
Online Access:Get full text
Tags: Add Tag
No Tags, Be the first to tag this record!
Description
Summary:Monitoring carnitine and acetylcarnitine levels in biological fluids is a powerful tool for diagnostic studies. Research has recently shown that the analysis of carnitine and related compounds in clinical samples can be accomplished by different analytical approaches. Because of the polar and ionic nature of the analytes and matrix complexity, accurate quantitation is a highly challenging task. Thus, sample processing factors, preparation/cleanup procedures, and chromatographic/ionization/detection parameters were evaluated. On the basis of the results obtained, a rapid, selective, sensitive method based on hydrophilic interaction liquid chromatography–tandem mass spectrometry for the analysis of carnitine and acetylcarnitine in serum and urine samples is proposed. The matrix effect was assessed. The proposed approach was validated, the limits of detection were in the nanomolar range, and carnitine and acetylcarnitine levels were found within the micromolar range in both types of sample. Figure Experimental workflow for the carnitine and acetylcarnitine determination in biological samples by HILIC-MS/MS
ISSN:1618-2642
1618-2650
DOI:10.1007/s00216-013-7193-6